LIM结构蛋白激酶1在肾透明细胞癌中的表达及其对肿瘤细胞增殖和迁移能力的影响  

Expression of Lim domain kinase 1 in renal clear cell carcinoma and effect on proliferation and migration of tumour cells

作　　者：郭文慧 袁红昌 GUO Wen-hui;YUAN Hong-chang(Department of Clinical Pharmacy,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453000,China)

机构地区：[1]新乡医学院第一附属医院临床药学学部,河南新乡453000

出　　处：《医药论坛杂志》2024年第7期699-704,共6页Journal of Medical Forum

摘　　要：目的本研究旨在探讨LIM结构蛋白激酶1(LIM domain kinase 1,LIMK1)在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)中的表达及其对肿瘤细胞增殖、迁移能力的影响。方法数据获取时间为2023年2月。通过癌症基因组图谱数据库TCGA获取ccRCC患者的转录组数据和临床信息,采用生存分析、单因素和多因素分析评估LIMK1对ccRCC患者预后的影响。利用功能富集分析预测LIMK1参与ccRCC病理过程的分子信号通路。通过RT-qPCR技术检测LIMK1在ccRCC细胞系中的表达水平,利用基因干扰技术敲减LIMK1在786-O细胞系中的表达。采用CCK8实验、划痕愈合实验的方法综合评估LIMK1对细胞增殖和迁移的影响。结果来自公共数据库的结果表明,在ccRCC组织中LIMK1呈现高表达(P<0.05),且高表达患者的预后较差(P<0.05)。单因素和多因素回归分析显示LIMK1是ccRCC患者的独立预后危险因素。功能富集分析揭示了LIMK1表达升高可能通过T细胞受体信号、P53信号、Toll样受体信号和JAK/STAT信号通路参与ccRCC的病理进程。实验结果表明,LIMK1在ccRCC细胞系中的表达水平增高,敲减LIMK1的表达能够抑制786-O细胞的增殖和迁移能力(P<0.05)。结论LIMK1是ccRCC患者不良预后的潜在诊断标志物,可通过调控肿瘤细胞增殖和侵袭等特性影响ccRCC的发生与发展。Objective The purpose of this study was to investigate the expression of LIM domain kinase 1(LIMK1)in clear cell renal cell carcinoma(ccRCC)and its effect on tumor cell proliferation and migration.Methods The data was obtained in February 2023.Transcriptome data and clinical information of ccRCC patients were obtained from The Cancer Genome Atlas(TCGA)database.The impact of LIMK1 on the prognosis of ccRCC patients was evaluated through survival analysis,univariate,and multivariate analysis.Using functional enrichment analysis to predict the molecular signaling pathways through which LIMK1 is involved in the pathogenesis of ccRCC.The expression level of LIMK1 in ccRCC cell lines was detected using RT-qPCR technology,and LIMK1 expression in 786-O cell lines was reduced using gene interference technology.Comprehensive assessments of the effects of LIMK1 on cell proliferation and migration,were conducted through CCK8 experiments,and scratch healing experiments.Results Results from the public database showed that LIMK1 was highly expressed in ccRCC tissues(P<0.05),and patients with high expression had a poorer prognosis(P<0.05).Univariate and multivariate regression analysis showed that LIMK1 was an independent prognostic risk factor for ccRCC patients.Functional enrichment analysis revealed that elevated LIMK1 expression might be involved in the pathological process of ccRCC through the T-cell receptor signaling,P53 signaling,Toll-like receptor signaling,and JAK/STAT signaling pathways.The experimental results indicate that the expression level of LIMK1 is increased in ccRCC cell lines,Knockdown of LIMK1 expression could significantly inhibit the proliferation and migration ability of 786-O cells(P<0.05).Conclusion LIMK1 is a potential diagnostic marker for poor prognosis and may influence the onset and progression of ccRCC by modulating properties such as tumour cell proliferation and invasion.

关 键 词：肾透明细胞癌 LIMK1 恶性生物学行为 

分 类 号：R737[医药卫生—肿瘤]