circ_0061140靶向miR-338-3p调控多发性骨髓瘤细胞的增殖和凋亡  

circ_0061140 regulates the proliferation and apoptosis of multiple myeloma cells through targeting miR-338-3p

作　　者：刘玉香 江庭秀[2] 汤杰 LIU Yuxiang;JIANG Tingxiu;TANG Jie(Department of Hematology,Liyang People′s Hospital,Liyang Jiangsu 213300;Department of Hematology,Fourth Affiliated Hospital of Guangxi Medical University,Liuzhou Guangxi 530021,China;Department of Oncology,Liyang People′s Hospital,Liyang Jiangsu 213300)

机构地区：[1]江苏省溧阳市人民医院血液科,213300 [2]广西医科大学第四附属医院血液科,广西柳州530021 [3]江苏省溧阳市人民医院肿瘤科,213300

出　　处：《蚌埠医学院学报》2024年第4期454-458,463,共6页Journal of Bengbu Medical College

摘　　要：目的:研究环状RNA 0061140(circ_0061140)对多发性骨髓瘤Sko-007细胞增殖和凋亡的影响和调控机制。方法:采用生物信息学数据库预测circ_0061140的靶miRNA,双荧光素酶报告实验证实circ_0061140与miR-338-3p靶向关系。实时定量PCR分析健康对照者和多发性骨髓瘤病人外周血中circ_0061140和miR-338-3p表达。将circ_0061140小干扰RNA(si-circ_0061140)、miR-338-3p模拟物、si-circ_0061140联合miR-338-3p抑制剂分别转染Sko-007,采用CCK-8法、流式细胞术分析干扰circ_0061140或过表达miR-338-3p或同时抑制circ_0061140和miR-338-3p对Sko-007细胞增殖、凋亡的影响。蛋白质印迹法分析B细胞淋巴瘤-2蛋白(Bcl-2)和Bcl相关x蛋白(Bax)表达变化。结果:miR-338-3p是circ_0061140达靶基因。circ_0061140在多发性骨髓瘤病人血清中的表达显著高于健康对照者(P<0.01),miR-338-3p在多发性骨髓瘤病人血清中的表达显著低于健康对照者(P<0.01)。干扰circ_0061140表达后Sko-007细胞增殖活力、Bcl-2表达显著降低(P<0.01),miR-338-3p和Bax表达、细胞凋亡率显著升高(P<0.01)。过表达miR-338-3p后Sko-007细胞增殖活力、Bcl-2表达显著降低(P<0.01),细胞凋亡率、Bax表达显著升高(P<0.01)。与干扰circ_0061140比较,同时抑制circ_0061140和miR-338-3p表达后Sko-007细胞增殖活力、Bcl-2表达显著升高(P<0.01),细胞凋亡率、Bax表达显著降低(P<0.01)。结论:circ_0061140通过靶向miR-338-3p促进多发性骨髓瘤细胞增殖,抑制细胞凋亡,在多发性骨髓瘤中具有致癌作用。Objective:To study the effect and regulatory mechanism of circular RNA 0061140(circ_0061140)on the proliferation and apoptosis of multiple myeloma Sko-007 cells.Methods:The bioinformatics database was used to predict the target miRNA of circ_0061140,and the dual luciferase report experiment was applied to confirm the targeting relationship between circ_0061140 and miR-338-3p.Expression of circ_0061140 and miR-338-3p in the peripheral blood of healthy controls and multiple myeloma patients was analyzed by real-time quantitative PCR.Circ_0061140 small interfering RNA(si-circ_0061140),miR-338-3p mimic,si-circ_0061140 combined with miR-338-3p inhibitor were transfected into Sko-007 cells respectively.The effects of interference with circ_0061140 or miR-338-3p overexpression or inhibition of circ_0061140 and miR-338-3p on Sko-007 cells proliferation and apoptosis were analyzed by CCK-8 method and flow cytometry.Expression of B-cell lymphoma-2 protein(Bcl-2)and Bcl-related x protein(Bax)was detected using Western blotting.Results:MiR-338-3p was the target gene of circ_0061140.The expression of circ_0061140 was significantly increased(P<0.01),while the the expression of miR-338-3p was significantly reduced(P<0.01)in in the serum of patients with multiple myeloma compared with healthy controls.The proliferation activity and Bcl-2 expression of Sko-007 cells were significantly reduced(P<0.01),and the expression of miR-338-3p and Bax,as well as cell apoptosis rate were significantly increased after interfering with circ_0061140(P<0.01).The proliferation activity and Bcl-2 expression of Sko-007 cells were significantly reduced(P<0.01),and the apoptosis rate and Bax expression were significantly increased after overexpression of miR-338-3p(P<0.01).Compared with interference circ_0061140,cell proliferation activity and Bcl-2 expression of Sko-007 were significantly increased(P<0.01),and the apoptosis rate and Bax expression were significantly reduced after simultaneously inhibiting the expression of circ_0061140 and miR-338-3

关 键 词：多发性骨髓瘤 环状RNA 0061140 细胞增殖 凋亡 miR-338-3p 

分 类 号：R733.3[医药卫生—肿瘤]