肠道微生物与焦虑关系的两样本孟德尔随机化研究  

A two-sample Mendelian randomized study on the relationship between intestinal microorganisms and anxiety

作　　者：付进杰 秦元 肖凌勇 戴晓矞[3,4] FU Jin-jie;QIN Yuan;XIAO Ling-yong;DAI Xiao-yu(Graduate School of Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;不详)

机构地区：[1]天津中医药大学研究生院,天津301617 [2]天津中医药大学中医学院,天津301617 [3]天津中医药大学第一附属医院,天津300380 [4]国家中医针灸临床医学研究中心,天津300380

出　　处：《现代预防医学》2024年第9期1537-1543,1579,共8页Modern Preventive Medicine

基　　金：国家重点研发计划(2022YFC3500501);天津市科技计划项目(18PTLCSY00050)。

摘　　要：目的利用孟德尔随机化探究211种肠道微生物与焦虑的因果关系。方法基于MiBioGen联盟和芬兰数据库中的全基因组关联研究数据集,提取符合条件的工具变量,以逆方差加权法为主要的孟德尔随机化分析方法,配合MR-Egger、Weighted median方法进行研究。最后通过Cochran Q统计量、MR-Egger回归的截距项、MR-PRESSO以及leave-one-out法对结果进行验证。结果蓝藻细菌门(OR=1.103,95%CI:1.001~1.215,P=0.048)、埃希-志贺菌属(OR=1.159,95%CI:1.027~1.309,P=0.017)、史雷克菌属(OR=1.170,95%CI:1.047~1.307,P=0.005)与焦虑风险呈正相关;厚壁菌门(OR=0.857,95%CI:0.754~0.974,P=0.018)、梭菌纲(OR=0.817,95%CI:0.701~0.951,P=0.009)、梭菌目(OR=0.849,95%CI:0.736~0.980,P=0.025)、真杆菌属(OR=0.861,95%CI:0.748~0.993,P=0.039)、瘤胃球菌_UCG004属(OR=0.886,95%CI:0.789~0.994,P=0.039)、瘤胃球菌_UCG011属(OR=0.919,95%CI:0.854~0.989,P=0.025)与焦虑风险呈负相关。所得结果通过验证,未发现异质性与水平多效性。结论本研究评估了肠道微生物与焦虑潜在的因果关系,为今后焦虑的诊断、预防与治疗等方面的相关研究提供参考。Objective To investigate the causal relationship between 211 intestinal microorganisms and anxiety by Mendelian randomization analysis.Methods Based on the genome-wide association study data of MiBioGen Alliance and Finnish database,the qualified instrumental variables were extracted,and the inverse variance weighting method was used as the main Mendelian randomized analysis method,combined with MR-Egger and Weighted median methods.Finally,the results were verified by Cochran’s Q statistics,intercept terms of MR-Egger regression,MR-PRESSO,and leave-one-out methods.Results Cyanobacteria(OR=1.103,95%CI:1.001-1.215,P=0.048),Shigella(OR=1.159,95%CI:1.027-1.309,P=0.017),and Slackia(OR=1.170,95%CI:1.047-1.307,P=0.017)were positively correlated with the risk of anxiety.Firmicutes(OR=0.857,95%CI:0.754-0.974,P=0.018),Clostridium(OR=0.817,95%CI:0.701-0.951,P=0.009),Clostridium(OR=0.849,95%CI:0.736-0.980,P=0.025),Eubacterium_rectale_group(OR=0.861,95%CI:0.748-0.993,P=0.039),Ruminococcaceae_UCG004(OR=0.886,95%CI:0.789-0.994,P=0.039),and Ruminococcaceae_UCG011(OR=0.919,95%CI:0.854-0.989,P=0.025)were negatively correlated with anxiety risk.The results were verified and no heterogeneity and horizontal pleiotropy were found.Conclusion This study evaluates the potential causal relationship between intestinal microorganisms and anxiety,providing reference for the diagnosis,prevention and treatment of anxiety in the future.

关 键 词：焦虑 肠道微生物 因果关联 孟德尔随机化 

分 类 号：R749.72[医药卫生—神经病学与精神病学]