结直肠癌病人外周血微RNA-4435、微RNA-762的表达及其与临床特征的相关性  

Expression of miR-4435 and miR-762 in peripheral blood of patients with colorectal cancer and their correlation with clinical characteristics

作　　者：曹砚杰 李琨琨 褚菲菲 吴慧丽 CAO Yanjie;LI Kunkun;CHU Feifei;WU Huili(Department of Gastroenterology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou,He'nan 450000,China)

机构地区：[1]郑州大学附属郑州中心医院消化内科,河南郑州450000

出　　处：《安徽医药》2024年第6期1152-1156,共5页Anhui Medical and Pharmaceutical Journal

基　　金：2022年河南省高等学校重点科研项目指导性计划项目(22B320010)。

摘　　要：目的观察结直肠癌病人外周血微RNA(miR)-4435、miR-762的表达情况,并分析其与临床特征、预后情况的相关性。方法选取2017年6月至2019年6月于郑州大学附属郑州中心医院就诊的100例疑似结直肠癌病人,经手术治疗后按照病理学检查结果分为结直肠癌组(64例)及非结直肠癌组(36例)。比较两组外周血miR-4435、miR-762表达水平;比较不同临床病理特征病人外周血miR-4435、miR-762表达水平;随访统计结直肠癌病人预后,将生存病人设为生存组,病死病人设为死亡组。比较两组外周血miR-4435、miR-762表达水平,采用Cox风险比例回归模型分析结直肠癌病人外周血miR-4435、miR-762表达水平与结直肠癌预后的相关性。结果与非结直肠癌组(0.61±0.08、0.89±0.12)比较,结直肠癌组外周血miR-4435、miR-762(0.96±0.12、1.35±0.24)表达水平升高(P<0.05)。与临床分期Ⅰ~Ⅱ期(0.84±0.11)、浸润深度T1~T2(0.71±0.09)、无淋巴结转移(0.79±0.11)病人比较,临床分期Ⅲ~Ⅳ期(1.03±0.15)、浸润深度T3~T4(1.25±0.15)、淋巴结转移(1.26±0.21)病人外周血miR-4435、miR-762表达水平升高(P<0.05)。随访至2022年6月,其中生存病人38例,病死病人26例,分别设置为生存组、死亡组。与生存组(0.87±0.09、1.12±0.14)比较,死亡组外周血miR-4435、miR-762(1.09±0.21、1.69±0.31)表达水平升高(P<0.05)。Cox风险比例回归分析显示,临床分期、浸润深度、淋巴结转移、miR-4435表达水平、miR-762表达水平均是结直肠癌预后不佳的危险因素(P<0.05)。结论结直肠癌病人外周血miR-4435、miR-762表达水平异常升高,与临床分期、浸润深度、淋巴结转移相关,且均为病人预后不佳的危险因素。Objective To observe the expression of peripheral blood microRNA(miR-4435 and miR-762)in patients with colorectal cancer,and to analyze their correlation with clinical characteristics and prognosis.Methods A total of 100 patients with suspected colorectal cancer who visited Zhengzhou Central Hospital Affiliated to Zhengzhou University from June 2017 to June 2019 were selected and divided into colorectal cancer group(64 cases)and non-colorectal cancer group(36 cases)according to the pathological examination results after surgical treatment.The expression levels of miR-4435 and miR-762 in peripheral blood of the two groups were compared.The expression levels of miR-4435 and miR-762 in peripheral blood of patients with different clinicopathological characteristics were compared.Follow-up statistics of the prognosis of colorectal cancer patients were made,and the surviving patients were set as the survival group,and the dead patients were set as the death group.The expression levels of miR-4435 and miR-762 in peripheral blood between the two groups were compared,and the Cox proportional hazards regression model was used to analyze the correlation between the expression levels of miR-4435 and miR-762 in peripheral blood and the prognosis of colorectal cancer.Results Compared with the non-colorectal cancer group(0.61±0.08,0.89±0.12),the expression levels of miR-4435 and miR-762 in peripheral blood of the colorectal cancer group(0.96±0.12,1.35±0.24)were increased(P<0.05).Compared with patients with clinical stageⅠ-Ⅱ(0.84±0.11),depth of invasion T1-T2(0.71±0.09),and no lymph node metastasis(0.79±0.11),the expression levels of miR-4435 and miR-762 in peripheral blood of patients with clinical stageⅢ-Ⅳ(1.03±0.15),depth of invasion T3-T4(1.25±0.15),and lymph node metastasis(1.26±0.21)were increased(P<0.05).During the follow-up until June 2022,38 patients survived and 26 patients died,which were set as the survival group and the death group,respectively.Compared with the survival group(0.87±0.09,1.12±0.14

关 键 词：结直肠肿瘤 微RNA-4435 微RNA-762 临床特征 预后 

分 类 号：R735.34[医药卫生—肿瘤]