The Effect of Spironolactone Loading on the Properties of 3D-Printed Polycaprolactone/Gold Nanoparticles Composite Scaffolds for Myocardial Tissue Engineering  

作　　者：Sharareh Ghaziof Shahrokh Shojaei Mehdi Mehdikhani Mohammad Khodaei Milad Jafari Nodoushan 

机构地区：[1]Department of Biomedical Engineering,Central Tehran Branch,Islamic Azad University,Tehran,1469669191,Iran [2]Stem Cells Research Center,Tissue Engineering and Regenerative Medicine Institute,Central Tehran Branch,Islamic Azad University,Tehran,1469669191,Iran [3]Department of Biomedical Engineering,Faculty of Engineering,University of Isfahan,Isfahan,8174673441,Iran [4]Materials Engineering Group,Golpayegan College of Engineering,Isfahan University of Technology,Golpayegan,87717-67498,Iran [5]Hard Tissue Engineering Research Center,Tissue Engineering and Regenerative Medicine Institute,Central Tehran Branch,Islamic Azad University,Tehran,1469669191,Iran

出　　处：《Journal of Bionic Engineering》2024年第2期924-937,共14页仿生工程学报（英文版）

摘　　要：Engineered cardiac constructs(ECC)aid in the progression of regenerative medicine,disease modeling and targeted drug delivery to adjust and aim the release of remedial combination as well as decrease the side effects of drugs.In this research,polycaprolactone/gold nanoparticles(PCL/GNPs)three-dimensional(3D)composite scaffolds were manufactured by 3D printing using the fused deposition modeling(FDM)method and then coated with gelatin/spironolactone(GEL/SPL).Scanning electron microscopy(SEM)and Fourier transform-infrared spectroscopy(FTIR–ATR)were applied to characterize the samples.Furthermore,drug release,biodegradation,behavior of the myoblasts(H9C2)cell line,and cytotoxicity of the 3D scaffolds were evaluated.The microstructural observation of the scaffolds reported interconnected pores with 150–300µm in diameter.The 3D scaffolds were degraded significantly after 28 days of immersion in stimulated body fluid(SBF),with the maximum rate of GEL-coated 3D scaffolds.SPL release from cross-linked GEL coating demonstrated the excess of drug release over time,and according to the control release systems,the drug delivery systems(DDS)went into balance after the 14th day.In addition,cell culture study showed that with the addition of GNPs,the proliferation of(H9C2)was enhanced,and with GEL/SPL coating the cell attachment and viability were improved significantly.These findings suggested that PCL/GNPs 3D scaffolds coated with GEL/SPL can be an appropriate choice for myocardial tissue engineering.

关 键 词：POLYCAPROLACTONE Gold nanoparticles Drug delivery systems SPIRONOLACTONE Cell behavior MYOBLASTS 

分 类 号：R318.08[医药卫生—生物医学工程]