高稳定液态D-阿洛酮糖3-差向异构酶制剂研究  

Study on Highly Stable Liquid D-Allulose 3-Epimerase Enzyme Preparation

作　　者：韩亚宏 郭元亨 王小艳 丁长河[1] 周浩 冉小力 田芳 HAN Yahong;GUO Yuanheng;WANG Xiaoyan;DING Changhe;ZHOU Hao;RAN Xiaoli;TIAN Fang(Henan University of Technology,Zhengzhou Henan 450001,China;Nutrition&Health Research Institute,COFCO Corporation,Beijing 102209,China;COFCO Biotechnology Co.,Ltd.,Beijing 100005,China;National Engineering Research Center of Corn Deep Processing,Jilin COFCO Biochemistry Co.,Ltd.,Changchun Jilin 130033,China)

机构地区：[1]河南工业大学,河南郑州450001 [2]中粮营养健康研究院有限公司,北京102209 [3]中粮生物科技股份有限公司,北京100005 [4]吉林中粮生化有限公司玉米深加工国家工程研究中心,吉林长春130033

出　　处：《当代化工》2024年第3期712-716,721,共6页Contemporary Chemical Industry

基　　金：国家重点研发计划项目,玉米淀粉及其多元化衍生物产品精细化加工成套技术开发(项目编号:2021YFD2101002)。

摘　　要：为解决D-阿洛酮糖3-差向异构酶(DAE)衰减过快、贮运成本高的问题,拟开发一款具有良好存贮稳定性的DEA液态酶制剂。以半衰期为评价依据,通过单因素试验筛选出对半衰期影响显著的4个因素,并进一步利用响应曲面法对酶制剂的配方进行优化。结果表明:稳定DAE酶制剂的最佳配比为0.1mol·L^(-1)CaCl_(2)、3.96%海藻糖、41.68%葡萄糖、29.32%PEG-400。在此条件下,半衰期为292天,与模拟理论值的相对误差仅为0.17%。通过工艺优化,将DAE的储存时间大幅延长,提高了酶制剂的贮运性能。The aim of this paper was developing a liquid enzyme preparation of D-allulose 3-epimerase(DAE)with good storage stability.According the half-life evaluations,the key influencing factors were selected using a single factor test.Then the four major influencing factors were selected.Then enzyme preparation formulation was optimized using response surface methodology.The results showed that the optimal compounding process for stable DAE was 0.1mol·L^(-1)CaCl_(2),3.96%trehalose,41.68%glucose,and 29.32%PEG-400.Under these conditions,the half-life was extended to 292 d.The relative error between the simulated value and true value was only 0.17%.This study extended the storage time of DAE through process optimization,significantly improved DAE storage and transportation performance.

关 键 词：D-阿洛酮糖3-差向异构酶 高稳定性 液态酶制剂 

分 类 号：TS202.3[轻工技术与工程—食品科学]