PI3K信号通路对食管鳞状细胞癌中OPN表达及EMT的影响  

Effect of PI3K signaling pathway on OPN expression and EMT in esophageal squamous cell carcinoma

作　　者：雷海鸣 陈湘 LEI Haiming;CHEN Xiang(College of Medical Jiangsu Vocational College of Medicine,Yancheng,Jiangsu 224005,China)

机构地区：[1]江苏医药职业学院医学院,江苏盐城224005

出　　处：《医药前沿》2024年第15期16-18,23,共4页Journal of Frontiers of Medicine

基　　金：江苏省盐城市医学科技发展计划项目(YK2021096)。

摘　　要：目的:研究PI3K信号通路在食管癌细胞中,对骨桥蛋白(OPN)表达调控的作用及上皮-间质转化(EMT)的影响。方法:将食管癌细胞EC-9706分为对照组、PI3K特异性抑制剂LY294002浓度5μM组、10μM及20μM组,经过6h处理后,采用反转录·聚合酶链反应(RT-PCR)检测OPN及EMT相关标记物E-钙黏蛋白(E-cadherin)mRNA表达的情况,以及Western blot检测各组OPN的蛋白情况。结果:LY294002处理后,可使食管癌细胞EC-9706的OPN mRNA和蛋白表达均受到抑制,随着LY294002浓度增大,抑制作用也进一步增大,呈现剂量依赖性关系,差异有统计学意义(P<0.01)。OPN与E-cadherin的mRNA表达呈负相关(r=-0.99,P<0.05)。结论:LY294002可以抑制食管癌细胞EC-9706中OPN的表达,OPN的表达及EMT受PI3K信号通路调控。Objective To study the role of PI3K signaling pathway in the regulation of osteopontin(OPN)expression and the impact of epithelial-mesenchymal transition(EMT)in esophageal cancer cells.Methods Divided esophageal cancer cells EC-9706 into a control group and a PI3K specific inhibitor LY 294002 concentration of 5μM group,10μM group and 20μM group.After 6 hours of treatment,reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the mRNA expression of OPN and EMT related marker E-cadherin,and Western blot was used to detect the protein expression of OPN in each group.Results After treatment with LY294002,both OPN mRNA and protein expression in esophageal cancer cells EC-9706 were inhibited.As the concentration of LY294002 increased,the inhibitory effect further increased,showing a dose-dependent relationship with statistical significance(P<0.01).The mRNA expression of OPN and E-cadherin was negatively correlated(r=-0.99,P<0.05).Conclusions LY294002 can inhibit the expression of OPN in esophageal cancer cells EC-9706.The expression of OPN and EMT are regulated by the PI3K signaling pathway.

关 键 词：食管鳞癌 骨桥蛋白 磷脂酰肌醇3-激酶 上皮-间质转化 

分 类 号：R571[医药卫生—消化系统]