基于TRAF2/AP-1信号通路探讨柴苓合剂对痰瘀互结MS-IR大鼠炎症因子影响  

Exploration of the Effect of Chailing Mixture(柴苓合剂)on MS-IR Rats with Phlegm Stasis Interaction Based on TRAF2/AP-1 Signal Pathway

作　　者：王晨阳 张业[1] WANG Chenyang;ZHANG Ye(Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046

出　　处：《辽宁中医药大学学报》2024年第6期37-42,共6页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：河南省重点研发与推广专项(科技攻关)项目(232102310459);河南省研究生教育改革与质量提升工程项目(YJS2022SZ20);河南省中医药科学研究专项课题(2022ZY1168);河南省特色骨干学科中医学第二批学科建设项目(STG-ZYX04-202138)。

摘　　要：目的基于TRAF2/AP-1信号通路探讨柴苓合剂对痰瘀互结代谢综合征胰岛素抵抗(MS-IR)大鼠糖代谢、脂代谢、炎症因子的部分作用机制。方法48只SPF级SD雄性大鼠随机分为空白组和造模组。造模组大鼠采用高脂高糖饲料喂养,注射小剂量链脲佐菌素(STZ)(35 mg/kg),同时用物理刺激方法,建立痰瘀互结型MSIR大鼠模型。造模成功后将大鼠随机分为模型组,柴苓合剂低、中、高剂量组,西药组进行干预。给药各组分别进行柴苓合剂、吡格列酮干预治疗3周,空白组、模型组给予等体积的蒸馏水灌服。生化检测大鼠空腹血糖(FBG)、空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);ELISA法检测血清肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)水平;Western Blot检测肝组织TRAF2、JNK、AP-1蛋白表达;HE染色切片,光学显微镜观察用药前后肝细胞损伤、肝细胞坏死、脂质堆积、炎症细胞浸润等情况。结果(1)糖代谢指标水平。与空白组相比,模型组大鼠FBG、FINS、HOMA-IR水平均升高(P<0.01);与模型组相比,柴苓合剂低、中、高剂量组及西药组FBG、FINS、HOMA-IR水平均降低(P<0.01);与西药组比较,柴苓合剂高剂量组FBG、FINS、HOMA-IR水平差异无统计学意义(P>0.05);与柴苓合剂低剂量组比较,柴苓合剂高、中剂量组及西药组FBG、FINS、HOMA-IR水平均降低(P<0.01);与柴苓合剂中剂量组比较,柴苓合剂高剂量组及西药组FBG、FINS、HOMA-IR水平降低(P<0.01)。(2)脂代谢指标水平。与空白组相比,模型组大鼠甘油三酯(TG)、胆固醇(CHO)水平均升高(P<0.01);与模型组相比,柴苓合剂低、中、高剂量组及西药组TG、CHO水平均降低(P<0.01);与西药组相比,柴苓合剂中、高剂量组TG、CHO水平均降低(P<0.05,P<0.01),柴苓合剂低剂量组TG、CHO水平差异无统计学意义(P>0.05);与柴苓合剂低剂量组相比,柴苓合剂中、高剂量组TG、CHO水平均降低(P<0.01);与柴苓合剂中剂Objective Based on the TRAF2/AP-1 signaling pathway,to explore the partial mechanism of Chailing Mixture(柴苓合剂)on glucose metabolism,lipid metabolism and inflammatory factors in MS-IR rats with phlegm and blood stasis.Methods 48 SPF male SD rats were randomly divided into blank group and model group.Rats in the model group were fed with high-fat and high-sugar feed,injected with a small dose of STZ(35 mg/kg)and physically stimulated to establish a MS-IR rat model with phlegm and blood stasis.After the successful modeling,the rats were randomly divided into the model group,the low-dose,medium-dose and high-dose groups of Chailing Mixture,and the Western medicine group for intervention.Each drug group received Chailing Mixture and pioglitazone intervention for 3 weeks,and the blank group and model group were given equal volumes of distilled water.Biochemical detection of FBG and FINS in rats,and calculation of HOMA-IR;detection of serum tumor necrosis factor(TNF-α)levels and interleukin 6(IL-6)by ELISA;detection of TRAF2,JNK,AP-1 protein expression in liver tissue by Western Blot;HE-stained sections were used to observe liver cell injury,liver cell necrosis,lipid accumulation,and inflammatory cell infiltration before and after treatment with an optical microscope.Results(1)The level of glucose metabolism indicators.Compared with the blank group,the levels of FBG,FINS,and HOMA-IR in the model group rats increased(P<0.01);Compared with the model group,the FBG,FINS,and HOMA-IR levels in the low,medium,and high dose groups of Chailing Mixture and the Western medicine group were all reduced(P<0.01);Compared with the Western medicine group,there was no statistically significant difference in FBG,FINS,and HOMA-IR levels in the high-dose group of Chailing Mixture(P>0.05);Compared with the low-dose group of Chailing Mixture,the FBG,FINS,and HOMA-IR levels in the high and medium dose groups of Chailing Mixture,as well as the Western medicine group,were all reduced(P<0.01);Compared with the medium-dose group of Chaili

关 键 词：代谢综合征 柴苓合剂 痰瘀互结型 TRAF2 JNK AP-1 TNF-α IL-6 

分 类 号：R289.5[医药卫生—方剂学]