基于转录组测序及生物信息学分析FNDC1基因在口腔鳞癌组织中的表达及意义  

Expression and significance of FNDC1 gene in oral cavity based on transcriptome sequencing and bioinformatics analysis

作　　者：张晓燕 张诗晗 李海洋 马行悦 李曼 刘文静 李向军[1] ZHANG Xiaoyan;ZHANG Shihan;LI Haiyang;MA Xingyue;LI Man;LIU Wenjing;LI Xiangjun(School and HospitalStomatology Hebei Medical University,Hospital Of Stomatology Hebei Medical University·Preventive Dentistry,Hebei Key Laboratory of Stomatology,The Oral Disease Clinical Medicine Research Institution of Hebei,Shijiazhuang 050017)

机构地区：[1]河北医科大学口腔医学院·口腔颌面外科,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,050017

出　　处：《现代口腔医学杂志》2024年第2期81-87,共7页Journal of Modern Stomatology

基　　金：河北省卫生健康委员会重点科技研究计划(20230183);河北省省级科技计划项目(21377719D)。

摘　　要：目的探讨FNDC1基因在口腔鳞状细胞癌(OSCC)中的表达水平及功能。方法采用生物信息学、转录组测序和细胞实验,检测FNDC1在OSCC中的表达水平及其在OSCC中可能的作用机制。结果FNDC1在OSCC组织中高表达(P<0.05),FNDC1表达的曲线下面积(AUC)为0.804,表明FNDC1与OSCC诊断之间的强相关性。同时还发现FNDC1的表达水平与吸烟(P<0.05)、临床分期(P<0.001)和病理分期(P<0.001)相关。FNDC1与细胞恶变具有较高的相关性,并且在成纤维细胞、肌成纤维细胞具有更高水平的表达。FNDC1与多种免疫细胞具有较高的相关性。结论本研究证实FNDC1在OSCC中高表达,且与OSCC的进展相关。Objective To investigate the expression level and function of FNDC1 in oral squamous cell carcinoma(OSCC).Methods Using bioinformatics,transcriptome sequencing,and cell experiments,the expression level of FNDC1 in OSCC and its possible mechanism of action in OSCC were detected.Results FNDC1 was highly expressed in OSCC tissue(P<0.05),and the area under the curve(AUC)of FNDC1 expression was 0.804,indicating a strong correlation between FNDC1 and OSCC diagnosis.It was also found that the expression level of FNDC1was correlated with smoking(P<0.05),clinical staging(P<0.001),and pathological staging(P<0.001).FNDC1 is highly correlated with cell malignancy and has higher levels of expression in fibroblasts and myofibroblasts.FNDC1 has a high correlation with various immune cells.Conclusion This study confirms that FNDC1 is highly expressed in OSCC and is associated with the progression of OSCC.

关 键 词：口腔鳞状细胞癌 FNDC1 生物信息学 

分 类 号：R739.8[医药卫生—肿瘤]