重庆市南岸区临床和食品来源空肠弯曲菌耐药特征及基因组分析  

Antimicrobial resistance and genomic characteristics of Campylobacter jejuni isolates from clinical and food sources in Nan'an District,Chongqing

作　　者：冯莉 宗华 李彩云 龚玲玉 罗益 周思雨 FENG Li;ZONG Hua;LI Caiyun;GONG Lingyu;LUO Yi;ZHOU Siyu(Department of Microbiology,Chongqing Nan'an Center for Disease Control and Prevention,Chongqing 400000,China)

机构地区：[1]重庆市南岸区疾病预防控制中心微生物检验科,重庆400000

出　　处：《中国国境卫生检疫杂志》2024年第2期205-210,共6页Chinese Journal of Frontier Health and Quarantine

基　　金：重庆市公共卫生重点专科(学科)建设经费资助项目。

摘　　要：目的了解重庆市南岸区临床和食品来源空肠弯曲菌的耐药特征及其分子流行特征并进行溯源分析,为该地区空肠弯曲菌感染的精准防控提供科学依据。方法对2021—2022年在重庆市南岸区6家哨点医院就诊的336例腹泻患者粪便样本和国家食品安全风险监测项目采集的60份生禽肉食品样本进行空肠弯曲菌分离鉴定,对分离的20株(15株临床株,5株食品株)空肠弯曲菌进行药敏分析、脉冲场凝胶电泳(PFGE)分型和全基因组测序,并下载公共数据库基因组进行比较;利用测序数据对菌株进行核心基因组多位点序列分型(cgMLST)、耐药基因分析,构建系统发育树。结果南岸区空肠弯曲菌临床株和食品株多重耐药率为70.00%;20株空肠弯曲菌分为19种MLST型别、19种cgMLST型别和18种PFGE带型;49株空肠弯曲菌(包括20株南岸区菌株和29株公共数据库菌株)系统发育树显示,南岸区分离自禽肉的4株食品株(2022NAF01、2022NAF02、2022NAF03、2022NAF04)与4株临床株(2021NAP02、2021NAP01、2021NAP03、2022NAP04)进化距离较近,6株与北京市4株菌距离接近。结论重庆市南岸区空肠弯曲菌的流行株呈多态性,在遗传特征上表现为多样性,且呈现很高的多重耐药性。Objective To analyze the antimicrobial resistance and genomic characteristics of Campylobacter jejuni strains isolated from human and food sources in Nan'an District of Chongqing,and conduct the traceability analysis to provide scientific basis for precise prevention and control of Campylobacter jejuni infection in the region.Methods The strain of Campylobacter jejuni was isolated and identified from fecal samples of 336 diarrhea patients treated at 6 sentinel hospitals in Nan'an District of Chongqing,and 60 raw poultry meat samples collected by the National Food Safety Risk Monitoring Project from 2021 to 2022.20 isolated strains of Campylobacter jejuni(15clinical strains and 5 food strains)were subjected to drug sensitivity testing,pulsed-field gel electrophoresis(PFGE)typing,whole genome sequencing and comparison with the public database genomes.The sequencing data was then used to perform core genome multilocus sequence typing(cgMLST)of the strains,drug-resistant genes analysis.A phylogenetic tree of 20 strains from Nan'an District and 29 strains from the public database was constructed.Results The multiresistance rate of clinical and food strains of Campylobacter jejuni in Nan'an District was 70.00%.The 20strains of Campylobacter jejuni were categorized into 19 MLST types,19 cgMLST types and 18 PFGE bands.The phylogenetic analysis showed that the evolutionary distance between the 4 food strains(2022NAF01,2022NAF02,2022NAF03,2022NAF04)isolated from poultry in Nan'an District and the 4 clinical strains(2021NAP02,2021NAP01,2021NAP03,2022NAP04)was closer,the evolutionary distance between the 6 strains in Nan'an District and 4 strains of Beijing was closer.Conclusion The prevalent strains of Campylobacter jejuni in Nanan District of Chongqing were polymorphic,with diversity in genetic characteristics and high multidrug resistance.

关 键 词：空肠弯曲菌 多重耐药 PFGE 全基因组测序 核心基因组多位点序列分型 

分 类 号：R183.4[医药卫生—流行病学]