Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments  

作　　者：姚静如 李国强 姚喜耀 周连杰 叶志凯 刘艳平 郑栋天 唐婷 宋克纳 陈果 刘雳宇 Jingru Yao;Guoqiang Li;Xiyao Yao;Lianjie Zhou;Zhikai Ye;Yanping Liu;Dongtian Zheng;Ting Tang;Kena Song;Guo Chen;Liyu Liu(Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials,College of Physics,Chongqing University,Chongqing 401331,China;Chongqing Key Laboratory for Resource Utilization of Heavy Metal Wastewater,Aquatic Ecosystems in the Three Gorges Reservoir Region of Chongqing Observation and Research Station,Chongqing University of Arts and Sciences,Yongchuan 402160,China;Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China;Department of Biomedical Engineering,Chongqing University of Posts and Telecommunications,Chongqing 400065,China;College of Medical Technology and Engineering,Henan University of Science and Technology,Henan 471023,China)

机构地区：[1]Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials,College of Physics,Chongqing University,Chongqing 401331,China [2]Chongqing Key Laboratory for Resource Utilization of Heavy Metal Wastewater,Aquatic Ecosystems in the Three Gorges Reservoir Region of Chongqing Observation and Research Station,Chongqing University of Arts and Sciences,Yongchuan 402160,China [3]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China [4]Department of Biomedical Engineering,Chongqing University of Posts and Telecommunications,Chongqing 400065,China [5]College of Medical Technology and Engineering,Henan University of Science and Technology,Henan 471023,China

出　　处：《Chinese Physics B》2024年第5期749-756,共8页中国物理B（英文版）

基　　金：support from the National Natural Science Foundation of China(Grant Nos.11974066,12174041,12104134,T2350007,and 12347178);the Fundamental and Advanced Research Program of Chongqing(Grant No.cstc2019jcyj-msxm X0477);the Natural Science Foundation of Chongqing(Grant No.CSTB2022NSCQMSX1260);the Science and Technology Research Program of Chongqing Municipal Education Commission(Grant No.KJQN202301333);the Scientific Research Fund of Chongqing University of Arts and Sciences(Grant Nos.R2023HH03 and P2022HH05);College Students’Innovation and Entrepreneurship Training Program of Chongqing Municipal(Grant No.S202310642002)。

摘　　要：Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and metastasis.However,conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration.Fortunately,the further development of microfluidic technology has provided solutions for the study of such questions.We utilize microfluidic chip to build a random collagen fiber microenvironment(RFM)model and an oriented collagen fiber microenvironment(OFM)model that resemble early stage and late stage breast cancer microenvironments,respectively.By combining cell culture,biochemical concentration gradient construction,and microscopic imaging techniques,we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells.The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model,with significant differences observed.Furthermore,we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model,with stronger inhibition on the high concentration side compared to the low concentration side.However,the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak.These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished.The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development,but also serve as a tool for the initial measurement of drug screening.

关 键 词：microfluidic chip random collagen fiber microenvironment(RFM) oriented collagen fiber microenvironment(OFM) cancer cell migration 

分 类 号：TN492[电子电信—微电子学与固体电子学] R73-3[医药卫生—肿瘤]