尿激酶型纤溶酶原激活因子调控人头颈部鳞状细胞癌转移的功能与机制研究  

STUDY ON THE FUNCTION AND MECHANISM OF PLAU IN REGULATING THE METASTASIS OF HUMAN HNSCC

作　　者：白艳萍 崔晓波[1] BAI Yanping;CUI Xiaobo(Department of Otolaryngology,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院耳鼻咽喉科,内蒙古呼和浩特010050

出　　处：《内蒙古医科大学学报》2024年第1期6-10,共5页Journal of Inner Mongolia Medical University

摘　　要：目的 探究尿激酶型纤溶酶原激活因子(urokinase-type plasminogen activator,PLAU)在头颈部鳞状细胞癌中的表达以及转移中的作用,并对其相关分子机制进行研究。方法 实验室培养人咽鳞癌细胞株FaDu细胞与人喉癌细胞株TU686细胞;使用siRNA靶向敲减/过表达PLAU基因后,利用qRT-PCR和Western Blot实验验证转染效率;Transwell与细胞划痕实验检测肿瘤细胞的侵袭、迁移能力变化情况;检测N-钙黏蛋白、锌指转录蛋白、波形蛋白在HNSCC细胞中表达的情况,分析其表达与PLAU对肿瘤远处转移的相关性。结果 PLAU在HNSCC中高表达,组间差异有统计学意义(P<0.05),且晚期或复发转移性HNSCC中的PLAU明显高表达于早期或原位癌。过表达PLAU有促进喉癌细胞转移的能力,PLAU可能调控N-钙黏蛋白、锌指转录蛋白、波形蛋白等诱导头颈部肿瘤的转移组间差异有统计学意义(P<0.05)。结论 PLAU可能是通过细胞-间充质转化过程促进HNSCC的迁移和侵袭。Objective This topic is to study the expression of PLAU in head and neck squamous cell carcinoma and its role in metastasis,and to study its related molecular mechanism.Methods Cultivate human pharyngeal squamous cell carcino-ma cell line FaDu and human laryngeal cancer cell line TU686 in the laboratory.After targeted knockdown/overexpression of the PLAU gene using siRNA,the transfection efficiency was validated using qRT-PCR and Western Blot experiments;Tran-swell and cell scratch assay were used to detect changes in the invasion and migration ability of tumor cells;Detect the expres-sion of N-cadherin,zinc finger transcription protein,and vimentin in HNSCC cells,and analyze their correlation with PLAU in distant tumor metastasis.Results PLAU was highly expressed in HNSCC,and the difference between the groups was statisti-cally significant(P<0.05),and PLAU in advanced or recurrent metastatic HNSCC was significantly overexpression in early or Carcinoma in situ.The overexpression of PLAU has the ability to promote the metastasis of laryngeal cancer cells.PLAU may regulate the metastasis of head and neck tumors induced by N-cadherin,zinc finger transcription protein,Vimentin,etc.The difference between groups is statistically significant(P<0.05).Conclusions PLAU may promote the migration and invasion of HNSCC induced cell mesenchymal transition.

关 键 词：头颈部鳞状细胞癌 尿激酶型纤溶酶原激活因子 转移 

分 类 号：R739.91[医药卫生—肿瘤]