电针联合神经减压/地塞米松对面神经受损家兔面神经病变及神经生长因子、Caspase-3表达的影响  

Effects of electroacupuncture combined with nerve decompression/dexamethasone on facial neuropathy,and expressions of nerve growth factors and Caspase-3 in rabbits with facial nerve injury

作　　者：向兴刚[1] 买买江·阿不力孜[1] 于晓晨[1] 蔡宁[1] 李大志[1] 张永辉[1] 依马木·依达依吐拉[1] 林琳[1] XIANG Xinggang;Maimaijiang Abulizi;YU Xiaochen;CAI Ning;LI Dazhi;ZHANG Yonghui;Yimamu Yida Yitula;LIN Lin(Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital,Urumqi,Xinjiang 830000)

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830000

出　　处：《现代中西医结合杂志》2024年第8期1047-1052,1136,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2020D01C134)。

摘　　要：目的观察电针联合神经减压/地塞米松对面神经损伤家兔面神经病变及血清神经生长因子(NGF)水平、面神经纤维组织中Caspase-3蛋白表达的影响,探讨其作用机制。方法将120只健康家兔随机分为假手术组、假手术+西医组、假手术+中医组、假手术+中西医组、模型组、模型+西医组、模型+中医组、模型+中西医组,每组15只。除假手术各组外,其余组均制备面神经损伤模型。术后假手术组、模型组不给予干预,假手术+西医组和模型+西医组均每天给予地塞米松1 mg/kg肌肉注射及面神经减压,假手术+中医组和模型+中医组均每天给予电针治疗(每次30 min,连续电针5 d休息2 d为1个周期),假手术+中西医组和模型+中西医组均每天给予地塞米松肌肉注射、面神经减压及电针治疗,各组均干预至术后第21天。分别于术后第1,7,21天检测外周血中NGF水平,HE染色观察面神经病变情况,免疫组化法检测面神经组织中Caspase-3阳性表达情况。结果术后第1,7,21天,模型各组血清NGF水平均明显低于假手术各组(P均<0.05);模型+中西医组血清NGF水平均明显高于模型组、模型+西医组、模型+中医组(P均<0.05),模型+西医组、模型+中医组均明显高于模型组(P均<0.05),模型+西医组与模型+中医组比较差异均无统计学意义(P均>0.05)。术后第1天,模型各组的Caspase-3阳性表达强度评分均明显高于假手术各组(P均<0.05);模型各干预组术后第1,7,21天的Caspase-3阳性表达强度评分均明显高于相应假手术各干预组(P均<0.05);模型+中西医组术后第1天和第7天的Caspase-3阳性表达强度评分均明显低于同期模型组和模型+西医组(P均<0.05),与模型+中医组比较差异均无统计学意义(P均>0.05);模型各组间术后第21天的Caspase-3阳性表达强度评分比较差异均无统计学意义(P均>0.05)。结论电针联合神经减压/地塞米松有促进面神经损伤修复的作用,其机�Objective It is to observe the effects and its mechanism of electroacupuncture combined with nerve decompression/dexamethasone on facial neuropathy,serum levels of nerve growth factors and expression of Caspase-3 protein in nerve fiber tissue in rabbits with facial nerve injury.Methods One hundred and twenty healthy rabbits were randomly divided into sham operation group,sham operation+western medicine(WM)group,sham operation+traditional Chinese medicine(TCM)group,sham operation+WM+TCM group,model group,model+WM group,model+TCM group,model+WM+TCM group,with 15 rabbits in each group.Facial nerve injury models were prepared in all groups except for the sham operation groups.The sham operation group and model group were not given any intervention after treatment,the sham operation+WM group and model+WM group were given dexamethasone 1 mg/kg by intramuscular injection and nerve decompression every day,the sham operation+TCM group and model+TCM group were treated with electroacupuncture(30 minutes per time,continuously treated for 5 days and rest for 2 days as one treatment course),the sham operation+WM+TCM group and model+WM+TCM group were treated with dexamethasone by intramuscular injection,nerve decompression and electroacupuncture,each group were continuously treated until the 21 th day after operation.On the 1 st,7 th,and 21 st day after surgery,the levels of NGF in peripheral blood were detected,the facial nerve lesions were observed by HE staining,and the positive expressions of Caspase-3 in facial nerve tissues was detected by immunohistochemistry.Results On the 1 st,7 th,and 21 st day after surgery,the serum levels of NGF in each model group were significantly lower than those in each sham operation group(all P<0.05),the serum level of NGF in the model+WM+TCM group was significantly higher than those in the model group,model+WM group and model+TCM group(all P<0.05),the model+WM group and model+TCM group were significantly higher than the model group(both P<0.05),there was no significant difference between th

关 键 词：面神经损伤 电针 神经减压 CASPASE-3蛋白 

分 类 号：R-332[医药卫生]