月桂酰精氨酸乙酯胞内抑菌机制及抗消化道蛋白酶水解研究  

Intracellular antibacterial mechanism of lauroyl arginate ethyl and its tolerance to gastrointestinal protease hydrolysis

作　　者：李佳伶 李媛媛 蒋思雨 张卓 郝刚[1] LI Jialing;LI Yuanyuan;JIANG Siyu;ZHANG Zhuo;HAO Gang(College of Food Science and Technology,Southwest Minzu University,Chengdu 610041;Chengdu AoKe Biotech Limited Liability Company,Chengdu 610041)

机构地区：[1]西南民族大学食品科学与技术学院,成都610041 [2]成都傲科新技术有限责任公司,成都610041

出　　处：《中国食品添加剂》2024年第5期66-74,共9页China Food Additives

基　　金：中央高校基本科研业务费优秀学生培养项目(项目编号:2021NYYXS35)。

摘　　要：本文通过对月桂酰精氨酸乙酯(LAE)的抑菌谱、细菌细胞胞内的抑菌机制以及耐受消化道蛋白酶水解的研究发现,LAE对G+菌、G-菌、真菌均有广谱抑菌活性,且对G+菌的抑菌活性普遍高于G-菌和真菌。LAE在凝胶阻滞实验中可以与菌体基因组强烈结合,随浓度增加阻滞现象更明显。LAE能抑制胞内蛋白的合成,使得碱性磷酸酶、β-半乳糖苷酶表达活性降低。LAE对胃蛋白酶和胰蛋白酶的耐受能力都很差,胃蛋白酶可部分水解LAE,胰蛋白酶能完全水解LAE,证明LAE可被消化道内蛋白酶水解,不会在肠道内保留。The antibacterial spectrum of lauroyl arginate ethyl(LAE),its intracellular antibacterial mechanism and its tolerance to protease hydrolysis in gastrointestinal tract were studied.The results showed that LAE had broad-spectrum antibacterial activity against Gram-positive,Gram-negative,and antifungal activity.Its antibacterial activity against Gram-positive bacteria was generally higher than that against Gram-negative bacteria and fungi.The gel retardation assay showed that LAE could strongly bind to bacterial genome.This retardation phenomenon was more obvious with the increase of concentration of LAE.LAE could inhibit the synthesis of intracellular proteins and reduce the expression activities of alkaline phosphatase andβ-galactosidase.LAE had poor tolerance to pepsin and trypsin and could be partly hydrolyzed by pepsin and completely hydrolyzed by trypsin.It was proved that LAE could be hydrolyzed by gastrointestinal protease and could not be retained in the intestine.

关 键 词：月桂酰精氨酸乙酯 抑菌谱 胞内抑菌机制 蛋白酶水解 

分 类 号：TQ465.91[化学工程—制药化工]