响应面法优化亚临界水提取艾草总黄酮的工艺研究  

Study on the process optimization of subcritical water extraction of total flavonoids from mugwort using response surface methodology

作　　者：张换平[1,2] 张怡怡 ZHANG Huanping;ZHANG Yiyi(School of Chemical and Environmental Engineering,Anyang Institute of Technology,Anyang 455000;Henan Key Laboratory of Subcritical High-Efficiency Extraction,Anyang Institute of Technology,Anyang 455000)

机构地区：[1]安阳工学院化学与环境工程学院,安阳455000 [2]安阳工学院河南省亚临界高效萃取重点实验室,安阳455000

出　　处：《中国食品添加剂》2024年第5期134-140,共7页China Food Additives

基　　金：河南省亚临界高校萃取重点实验室;河南省大中专院校就业创业课题(JYB2023077);安阳市重大科技专项项目(2022A02SF001)。

摘　　要：借助于响应面法探究亚临界水提取艾草总黄酮的最佳工艺条件。在考察提取温度、提取时间、液料比以及磁力搅拌转速等单因素条件对艾草总黄酮提取率影响的基础上,用Design Expert软件对提取工艺进一步优化,建立亚临界水提取艾草总黄酮的多元二次回归方程,通过拟合回归分析得到最佳提取工艺条件为:提取温度127.3℃、提取时间20.8 min、液料比87.7∶1 mL/g,艾草总黄酮提取率的预测值15.28%与验证值基本吻合,说明该模型具有一定的可行性,能够较好的预测艾草黄酮的提取率。采用红外光谱及液相色谱-质谱联用技术对艾草总黄酮进行结构表征,通过综合分析,推测艾草总黄酮中可能含有大豆素。该提取工艺简单可靠,经济环保,可为黄酮类物质的提取提供一种可参考的方法。A subcritical water extraction process was optimized by response surface experiments to extract totalflavonoids from mugwort.Based on tests of single factors including extraction temperature,extraction time,liquid-solid ratio,and magnetic stirring speed,Design Expert13.0 software was used to design the extraction process optimization experiment.Response surface interaction analysis was used to establish multiple quadratic regression equation for subcritical water extraction of total flavonoids from mugwort.The optimal extraction conditions were obtained by regression analysis.The optimal extraction process of total flavonoids from mugwort was as follows:extraction time 20.8 min,extraction temperature 127.3℃,liquid-solid ratio 87.7:1 mL/g.The predicted value of 15.28%of mugwort totalflavonoid extraction rate was very close with the experiment data,which indicated the model had certain feasibility.The structure of totalflavonoids in mugwort was characterized by FT-IR and LC-MS,the results showed that the mugwort totalflavonoids might contain daidzein.The extraction process was simple and reliable,economical,and environmentally friendly.The study provides a reference forflavonoids extraction.

关 键 词：响应面法 亚临界水 提取 艾草 黄酮 

分 类 号：TS202.1[轻工技术与工程—食品科学] R284.2[轻工技术与工程—食品科学与工程]