不同方法提取姜黄油挥发性化学成分GC-MS分析  

GC-MS analysis of volatile chemical components of turmeric oil extracted with different methods

作　　者：张玲玲 黄幼霞 蔡聪艺 张文州 ZHANG Lingling;HUANG Youxia;CAI Congyi;ZHANG Wenzhou(Quanzhou Medical College,Quanzhou 362000)

机构地区：[1]泉州医学高等专科学校,泉州362000

出　　处：《中国食品添加剂》2024年第5期302-311,共10页China Food Additives

基　　金：泉州市科技计划项目(2021C046R)。

摘　　要：采用同时蒸馏萃取法(SDE),选择石油醚、正己烷、乙醚3种萃取剂及纤维素酶、盐析2种辅助方式共五种方式提取姜黄油,比较不同提取方法对姜黄油挥发性化学成分的影响。利用GC-MS法鉴定姜黄油的挥发性化学成分,归一化法计算挥发性化学成分相对百分含量,并对数据进行主成分和聚类分析。结果表明:用5种提取方法共鉴定出84种化合物,其中共有化合物24种。主成分分析结果显示4种主成分可反映姜黄油挥发性化学成分的100%的信息,芳姜黄酮、姜黄酮、β-姜黄酮、β-倍半水芹烯和α-姜油烯对姜黄风味贡献最大。聚类分析结果表明,5种提取方法可分为3类即T1和T2一类,T4一类,T3和T5一类。24种化合物被聚为2类,其中α-姜油烯等5种含量最多的化合物聚为一类。不同提取方法对姜黄油的提取效果影响较显著,以纤维素酶辅助SDE提取方式和盐析辅助SDE提取方式更为明显。To compare the effects of different extraction methods on volatile components of turmeric oil,simultaneous distillation extraction(SDE)was used to extract the turmeric oil,3 different extracts of petroleum ether,n-hexane and ether extractants,and 2 different auxiliary methods with cellulase and salting precipitation respectively were selected for the turmeric oil extraction.The volatile chemical component of the extracted turmeric oil was identified by GC-MS,and the relative percentage content of the volatile chemical component was calculated using normalization method.The data was analyzed by principal component analysis and cluster analysis.The results showed that a total of 84 compounds were identified and 24 were found in the extracted turmeric oil by all 5 extraction methods.The principal component analysis showed that four principal components represented 100%of the volatile chemical components in the extracted turmeric oil.The ar-turmerone,turmeone,β-turmerone,β-sesquiphellandrene,andα-zingiberene contributed the most to theflavor of turmeric.The cluster analysis showed thatfive extraction methods were divided into three categories:T1 and T2,T4,T3 and T5.Twenty-four compounds were clustered into two groups,α-gingerene and the otherfive most abundant components were clustered into one group.Different extraction methods significantly affected extraction efficiency of turmeric volatile oil,cellulase-assisted SDE and salt-assisted SDE had better extraction results.

关 键 词：蒸馏萃取法 GC-MS 主成分分析 聚类分析 酶辅助 盐析 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]