桑树桑黄液体发酵及多糖提取工艺优化  

Optimization of Liquid Fermentation and Polysaccharide Extraction of Sanghuangporus sanghuang

作　　者：李德毓 司豆豆 彭浩[1,2] 曹伟刚 郑晓芹 张升明 LI Deyu;SI Doudou;PENG Hao;CAO Weigang;ZHENG Xiaoqin;ZHANG Shengming(School of Biolgical Science and Engineering,Shaanxi University of Technology,Hanzhong 723001,China;Shaanxi Hanzhong City Science and Technology Resources Coordination Center,Hanzhong 723001,China;Qinba Ecological Protection Center,Hanzhong City,Shaanxi Province,Hanzhong 723001,China;Shaanxi Xixiang County Mushroom Research Institute,Shaanxi Hanzhong 723500,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723001 [2]陕西省汉中市科技资源统筹中心,陕西汉中723001 [3]陕西省汉中市秦巴生态保护中心,陕西汉中723001 [4]陕西省西乡县食用菌研究所,陕西汉中723500

出　　处：《黑龙江农业科学》2024年第6期64-70,共7页Heilongjiang Agricultural Sciences

基　　金：陕西省教育厅科技计划项目(20JS024);陕南秦巴山区生物资源综合开发协同创新中心项目(SLGPT2019KF02-01);秦巴生物资源与生态环境省部共建国家重点实验室(培育)“市校共建”科研专项(SXC-2307);西乡县技术创新和新产品试制项目(XXKJ-2021-02)。

摘　　要：桑树桑黄是一种具有良好药效的药用多孔菌,其多糖是主要活性成分之一,具有抗肿瘤、抗氧化、免疫调节和其他药理作用。为提高桑树桑黄菌丝体干重及桑黄多糖得率,采用单因素和正交试验对影响桑黄菌丝体干重的培养基配方及液体发酵培养条件进行优化;利用超声波辅助提取法对桑黄多糖提取工艺条件进行优化。结果表明,桑树桑黄最佳培养基配方为马铃薯(去皮)200 g·L^(-1)、乳糖32 g·L^(-1),胰蛋白胨10 g·L^(-1),NaCl 2.00 g·L^(-1),MgSO 4·7H 2 O 1 g·L^(-1),VB 110.0 mg·L^(-1),获得菌丝体干重为2.82 g·L^(-1)。在温度28℃,接种量9%,转速180 r·min-1,pH6.0条件下,最大菌丝量为3.12 g·L^(-1)。桑黄多糖最佳提取工艺为超声波功率200 W,料液比1∶60 g·mL^(-1),提取时间40 min,温度50℃,此时桑黄多糖得率为5.54%。说明本研究优化的桑树桑黄培养基配方、发酵条件及多糖提取工艺合理、可行。Sanghuangporus sanghuang is a medicinal porous bacterium with good medicinal efficacy,and its polysaccharide is one of the main active ingredients,with antitumor,antioxidant,immunomodulatory and other pharmacological effects.In order to improve the dry weight of S.sanghuang mycelia and the yield of S.sanghuang polysaccharide,single factor and orthogonal test were used to optimize the medium formula and liquid fermentation culture conditions affecting the dry weight of S.sanghuang mycelia.Ultrasonic assisted extraction method was used to optimize the extraction conditions of polysaccharide yield of S.sanghuang.The results showed that the optimal medium formula of S.sanghuang was potatoes(peeled)200 g·L^(-1),lactose 32 g·L^(-1),tryptone 10 g·L^(-1),NaCl 2.00 g·L^(-1),MgSO 4·7H 2 O 1 g·L^(-1),VB 110.0 mg·L^(-1),and mycelium dry weight was 2.82 g·L^(-1).Under the conditions of temperature 28℃,inoculation amount 9%,rotational speed 180 r·min-1 and pH6,the maximum mycelium amount was 3.12 g·L^(-1).The optimal extraction process was as follows:ultrasonic power 200 W,solid-liquid ratio 1∶60,extraction time 40 min,temperature 50℃,the yield of polysaccharides was 5.54%.The results showed that the optimized culture medium formula,fermentation conditions and polysaccharide extraction technology of S.sanghuang were reasonable and feasible,which could provide reference for liquid fermentation of S.sanghuang mycelium to produce polysaccharide.

关 键 词：桑树桑黄 液体发酵 多糖 超声波辅助提取法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ460.6