miR-200b-3p accelerates progression of pituitary adenomas by negatively regulating expression of RECK  

作　　者：XIAOXI WANG YANFEI JIA QIANG LI QIANG YANG YINGFENG LIU BEIFENG WEI XIANG NIU YINJIE ZHANG XIAODONG LUO ZIYU ZHAO PENG WANG 

机构地区：[1]Department of Neurosurgery,Tianshui First People’s Hospital,Tianshui,China [2]Department of Neurosurgery,The Second Affiliated Hospital of Lanzhou University,Lanzhou,China

出　　处：《Oncology Research》2024年第5期933-941,共9页肿瘤学研究（英文）

基　　金：supported by Correlation between RECK and GH-type pituitary adenomas(No.21JR11RE027).

摘　　要：MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to provide a theoretical basis for treatment.Bioinformatics was used to analyze pituitary adenoma-related genes and screen new targets related to RECK and miRNA.As well,the relationship between miR-200b-3p and RECK protein was verified using a double-luciferase reporter gene assay.The expression of miR-200b-3p in clinical samples was analyzed by in situ hybridization.Transfection of the miR-200b-3p inhibitor and small interfering-RECK(si-RECK)was verified by qPCR.GH3 cell viability and proliferation were detected using CCK8 and EdU assays.Apoptosis was detected by flow cytometry and western blotting.Wound healing and Transwell assays were used to detect cell migration and invasion.The effects of miR-200b-3p and RECK on GH3 cells were verified using salvage experiments.miR-200b-3p was highly expressed in pituitary tumor tissue.Inhibitors of miR-200b-3p inhibited cell proliferation promoted cell apoptosis,inhibited invasion and migration,and inhibited the expression of matrix metalloproteinases.Interestingly,miR-200b-3p negatively regulated RECK.The expression of RECK in pituitary adenoma tissues was lower than that in neighboring tissues.Si-RECK rescued the function of miR-200b-3p inhibitors in the above cellular behaviors,and miR-200b-3p accelerated the development of pituitary adenoma by negatively regulating RECK expression.In summary,this study investigated the molecular mechanism by which miR-200b-3p regulates the progression of pituitary adenoma through the negative regulation of RECK.The findings provide a new target for the treatment of pituitary adenoma.

关 键 词：Pituitary adenomas miR-200b-3p RECK Matrix metalloproteinase 

分 类 号：R736.4[医药卫生—肿瘤]