Ctsk 基因敲除调控H型血管参与小鼠牙槽窝愈合的研究  

Study of Ctsk knockout regulating type H blood vessel formation and tooth extraction socket healing in mice

作　　者：张武阳 李登科 王一名 李元 陈禹喆 郑雪妮 周宏志 胡开进 薛洋 ZHANG Wuyang;LI Dengke;WANG Yiming;LI Yuan;CHEN Yuzhe;ZHENG Xueni;ZHOU Hongzhi;HU Kaijin;XUE Yang(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,The Third Hospital Affiliated of Air Force Military Medical University,China,710032 Xi'an;Department of Stomatology,Air Force Hospital of Southern Theater Command,Guangzhou)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学第三附属医院口腔颌面外科,西安710032 [2]南部战区空军医院口腔科

出　　处：《实用口腔医学杂志》2024年第3期330-336,共7页Journal of Practical Stomatology

基　　金：军事口腔医学国家重点实验室自主研究课题(编号:2019ZA09);陕西省重点研发计划项目(编号:2019SF-173)。

摘　　要：目的:观察组织蛋白酶K(CTSK)对小鼠牙槽窝愈合及H型血管生成的作用。方法:利用CRISP R/Cas9技术构建Ctsk基因敲除小鼠,通过基因测序、大体观、Micro-CT及免疫组化对基因敲除情况进行鉴定;随后选择8周龄野生(wide type,WT)和Ctsk基因敲除小鼠拔除左侧上颌第一磨牙,于术后第7、10、14、21、28和35天处死各组小鼠(n=3),利用体视显微镜观察牙槽窝软组织愈合情况,利用Micro-CT观察骨组织愈合情况,利用免疫荧光染色观察牙槽窝愈合过程H型血管生成情况。结果:Ctsk基因敲除不影响小鼠牙槽窝黏膜软组织愈合,但能显著促进牙槽窝骨质愈合,且Ctsk基因敲除小鼠牙槽窝内H型血管生成显著增多。结论:Ctsk基因敲除能够增强H型血管生成,进而促进小鼠牙槽窝骨质愈合。Objective:To study the effects of cathepsin K(CTSK)on the healing process of tooth extraction socket and type H blood vessel angiogenesis in mice.Methods:Ctsk knockout(Ctsk-/-)mice were generated by CRISPR/Cas9 technology,and genotype sequencing,general observation,Micro-CT and immunohistochemistry were performed to confirm successful knockout of Ctsk.Then 8 week-old WT and Ctsk-/-mice were used to establish the tooth extraction modle by extracting the left maxillary first molars,and the mice were sacrificed at the day 7,10,14,21,28 and 35 respectively(n=3)after tooth extraction.Then samples were subjected to stereo microscope and Micro-CT examination.Immunofluorescence staining was used to study the effect of Ctsk knockout on type H blood vessel angiogenesis.Results:Ctsk knockout did not affect the soft tissue healing of tooth extraction socket,but significantly promoted the bone healing process,and Ctsk deficency significantly enhanced type H blood vessel ang iogenesis in the tooth extraction socket.Conclusion:Ctsk knockout can enhance type H vessel angiogenesis,and promote bone healing process of tooth extraction socket in mice.

关 键 词：组织蛋白酶K 拔牙创愈合 H型血管 骨再生 

分 类 号：R782.1[医药卫生—口腔医学] R329.2[医药卫生—临床医学]