桔梗皂苷D对人结肠癌细胞中程序性细胞死亡配体-1表达和血管生成的影响  

Effects of platycodin D on programmed death ligand 1 expression and angiogenesis in human colon cancer cells

作　　者：李程程 李光亿 邰屹 刘欣哲 曹晟 马娟 LI Chengcheng;LI Guangyi;TAI Yi;LIU Xinzhe;CAO Sheng;MA Juan(Key Laboratory of Natural Medicines of Changbai Mountain,Ministry of Education,Molecular Medicine Research Center,Yanbian University College of Pharmacy,Yanji 133002,Jilin,China)

机构地区：[1]长白山天然药物教育部重点实验室延边大学药学院分子药物研究中心,吉林延吉133002

出　　处：《延边大学医学学报》2024年第1期1-5,共5页Journal of Medical Science Yanbian University

基　　金：吉林省大学生创新创业训练计划项目(编号:2023YDCXCY058);国家自然科学基金项目(81760657)。

摘　　要：[目的]探讨桔梗皂苷D(PLD)对人结肠癌细胞的程序性细胞死亡配体-1(PD-L1)表达、集落形成、伤痕愈合及内皮细胞血管生成的影响.[方法]采用MTT法检测PLD对人结肠癌细胞HCT116细胞增殖的影响,蛋白免疫印迹法检测PLD对HCT116细胞中PD-L1蛋白表达的影响,细胞集落法观察PLD对HCT116细胞增殖能力的影响,划痕实验法观察PLD对细胞迁移速率的影响,小管形成实验法观察PLD对内皮细胞血管生成的影响.[结果]PLD可抑制肿瘤细胞增殖(P<0.001),以浓度和时间依赖性地抑制HCT116细胞中PD-L1蛋白的表达,抑制HCT116细胞集落的形成、迁移和内皮细胞血管的生成.[结论]PLD可抑制结肠癌HCT116细胞中的PD-L1表达,抑制HCT116细胞的增殖、迁移及血管生成.OBJECTIVE To explore the effect of platycodin D(PLD)on programmed death ligand 1(PD-L1)expression,colony formation,wound healing and angiogenesis of endothelial cells in human colon cancer cells.METHODS MTT assay was used to detect the effect of PLD on the proliferation of human colon cancer cells HCT116.Western blot assay was used to detect the effect of PLD on the expression of PD-L1 protein in HCT116 cells.Cell colony formation experiment was used to observe the effect of PLD on the proliferation ability of HCT116 cells.Scratch assay was used to observe the effect of PLD on cell migration rate.Tubule formation assay was used to observe the effect of PLD on the angiogenesis of endothelial cells.RESULTS PLD could inhibit the proliferation of tumor cells(P<0.001),and inhibit the expression of PD-L1 protein in HCT116 cells in a concentration and time dependent manner.PLD could inhibit the cell colony formation and migration of HCT116 cells and angiogenesis of endothelial cells.CONCLUSION PLD can inhibit the expression of PD-L1 in human colon cancer HCT116 cells,and inhibit the proliferation,migration of HCT116 cells and angiogenesis.

关 键 词：桔梗皂苷D 结肠癌 PD-L1 血管生成 

分 类 号：R285.5[医药卫生—中药学]