正畸患者口腔细菌感染情况及对血清、龈沟液PAK5、IL-6、IL-8水平的影响  

Oral bacterial infection in orthodontic patients and its impact on serum and gingival crevicular fluid PAK5,IL⁃6,and IL⁃8 levels

作　　者：郭红燕 吴福焱 孙晓辉 田丽丽 GUO Hongyan;WU Fuyan;SUN Xiaohui;TIAN Lili(Dental Clinic,Liaocheng Fourth People's Hospital,Liaocheng,Shandong,China,252000)

机构地区：[1]聊城市第四人民医院口腔门诊,山东聊城252000

出　　处：《分子诊断与治疗杂志》2024年第5期953-957,共5页Journal of Molecular Diagnostics and Therapy

基　　金：山东省医药卫生科技发展计划项目(202007100610)。

摘　　要：目的分析正畸患者口腔细菌感染情况及对血清、龈沟液p2l活化激酶(PAK5)、白介素-6(IL-6)、白介素-8(IL-8)水平的影响。方法回顾性分析2020年12月至2022年12月间聊城市第四人民医院口腔科门诊收治的446例正畸治疗患者临床资料,分析治疗1周后患者口腔感染情况,并按是否感染分为感染组和非感染组,分离并培养感染组患者口腔感染病原菌,分析病原菌分布情况,并记录药敏试验结果。比较两组治疗前、治疗1周后龈沟液、血清IL-6、IL-8、PAK5水平,绘制ROC曲线评估龈沟液、血清IL-6、IL-8、PAK5预测口腔感染的效能。结果纳入患者中,感染45例,感染率为10.09%;45例正畸治疗口腔感染患者共培养分离出83株病原菌,有3例患者合并2种病原菌感染;革兰阳性菌38株,占比45.78%;革兰阴性菌45株,占比54.22%;病原菌分布中占比前三位是肺炎克雷伯菌、表皮葡萄球菌、牙龈卟啉单胞菌;革兰阳性菌中青霉素耐药率最高,革兰阴性菌中阿莫西林/克拉维酸耐药率最高。治疗前,两组龈沟液、血清IL-6、IL-8、PAK5水平比较差异均无统计学意义(P>0.05);治疗1周后,两组龈沟液、血清IL-6、IL-8、PAK5均高于治疗前,且感染组高于非感染组,差异有统计学意义(P<0.05)。ROC曲线显示:治疗后龈沟液、血清IL-6、IL-8、PAK5是预测正畸治疗口腔感染的有效指标(P<0.05)。结论正畸患者口腔感染病原菌中革兰阴性菌占比较高,监测正畸患者治疗1周后的龈沟液及血清IL-6、IL-8、PAK5水平有利于评估其口腔感染发生风险。Objective To analyze the oral bacterial infection status in orthodontic patients and its effects on p2l activated kinase(PAK5),interleukin⁃6(IL⁃6)and interleukin⁃8(IL⁃8)in serum and gingival crevicular fluid.Methods The clinical data of 446 patients undergoing orthodontic treatment at the Depart⁃ment of Stomatology at Liaocheng Fourth People's Hospital were retrospectively analyzed from December 2020 to December 2022.The oral infection status of patients after one week of treatment was assessed,and they were divided into an infection group and a non⁃infection group based on their infection status.Pathogenic bacteria causing oral infections in the infected group were isolated,cultured,and analyzed for distribution,as well as tested for drug sensitivity.Levels of IL⁃6,IL⁃8,and PAK5 in gingival crevicular fluid and serum were compared between the two groups before treatment and after one week of treatment.ROC curves were generat⁃ed to assess the effectiveness of IL⁃6,IL⁃8,and PAK5 in predicting oral infections based on their levels in gin⁃gival crevicular fluid and serum.Results Among the 446 enrolled patients,45 cases were infected,resulting in an infection rate of 10.09%.A total of 83 strains of pathogenic bacteria were cultured and isolated,with 3 patients identified as having dual infections with two strains of pathogenic bacteria.Of these strains,38 strains were Gram⁃positive bacteria,accounting for 45.78%,while 45 strains were Gram⁃negative bacteria.account⁃ing for 54.22%.The most common pathogenic bacteria identified were Klebsiella pneumoniae,Staphylococcus epidermidis and Porphyromonas gingivalis.The highest resistance rate among Gram⁃positive bacteria was to penicillin,while among Gram⁃negative bacteria,it was to amoxicillin/clavulanic acid.Prior to orthodontic treatment,there were no statistically significant differences in the levels of IL⁃6,IL⁃8,and PAK5 in gingival crevicular fluid and serum between the two groups(P>0.05).After one week of treatment,levels of IL⁃

关 键 词：正畸治疗 口腔感染 病原菌 白介素-6 白介素-8 p2l活化激酶 

分 类 号：R783.5[医药卫生—口腔医学]