血小板衍生生长因子A不同转录变体对膀胱癌细胞增殖和侵袭的影响  

Effects of different transcriptional variants of platelet-derived growth factor A on proliferation and invasion of bladder cancer cells

作　　者：周帅 余力 丁勇杰 李艳松 胡祎舜 刘敏豪 汤晨昊 刘子阳 彭袈豪 袁作洈 柳栩然 汪力 成聪 刘涛[2] 曾金敏[1] 廖义翔[1] 杨涛 Zhou Shuai;Yu Li;Ding Yongjie;Li Yansong;Hu Yishun;Liu Minhao;Tang Chenhao;Liu Ziyang;Peng Jiahao;Yuan Zuowei;Liu Xuran;Wang Li;Cheng Cong;Liu Tao;Zeng Jinmin;Liao Yixiang;Yang Tao(Department of Urology,Jingzhou Central Hospital,Jingzhou Hospital Affiliated to Yangtze University,Jingzhou 434020,China;Department of Urology,Wuhan University Zhongnan Hospital,Wuhan 430071,China)

机构地区：[1]荆州市中心医院长江大学附属荆州医院泌尿外科,荆州434020 [2]武汉大学中南医院泌尿外科,武汉430071

出　　处：《中华实验外科杂志》2024年第4期777-779,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨血小板衍生生长因子A(PDGFA)的不同转录变体PDGFA-L和PDGFA-S对膀胱癌细胞增殖和侵袭的影响。方法通过生物信息学方法对肿瘤基因组图谱(TCGA)中的外显子测序数据进行分析,筛选出膀胱癌中PDGFA发生的异常选择性剪接事件。在膀胱癌细胞株(EJ和T24)中分别过表达PDGFA-L和PDGFA-S。设置组别为EJ和T24细胞株对照组和稳定过表达PDGFA实验组的EJ(EJ-PDGFA-L组和EJ-PDGFA-S组)和T24胞株(T24-PDGFA-L组和T24-PDGFA-S组)。通过细胞计数试剂盒(CCK-8)实验、平板克隆形成实验、Transwell实验观察PDGFA对膀胱癌增殖与迁移的影响。采用独立样本t检验进行组间比较。结果通过生物信息学方法分析各肿瘤中PDGFA基因的外显子测序数据,可见PDGFA在多种癌症中发生异常选择性剪接,其中包括膀胱癌;与正常组织比较,肿瘤组织中PDGFA-L的表达高于PDGFA-S(P<0.05)。CCK-8结果显示,过表达PDGFA组细胞增殖能力明显高于对照组[EJ细胞株中PDGFA-L和PDGFA-S组吸光度值明显高于对照组(1.958±0.081比0.982±0.357,t=12.765,P<0.05)和(1.324±0.476比0.982±0.357,t=17.977,P<0.05);T24细胞株中PDGFA-L和PDGFA-S组吸光度值明显高于对照组(1.619±0.053比0.821±0.029,t=17.61,P<0.05)和(1.219±0.073比0.821±0.029,t=16.32,P<0.05)];集落形成实验结果显示,过表达PDGFA组细胞增殖能力明显高于对照组[EJ细胞株中PDGFA-L和PDGFA-S组集落数明显高于对照组(235.50±10.50比160.10±2.11,t=5.12,P<0.05)和(181.30±6.32比160.10±2.11,t=3.15,P<0.05);T24细胞株中PDGFA-L和PDGFA-S组集落数明显高于对照组(590.0±27.0比351.0±16.0,t=6.713,P<0.05)和(527.0±23.3比351.0±16.0,t=6.907,P<0.05)];Transwell实验结果显示,过表达PDGFA组细胞迁移能力明显高于对照组[EJ细胞株中PDGFA-L和PDGFA-S组迁移数明显高于对照组(635.0±15.0比125.0±7.5,t=17.32,P<0.05)和(611.0±19.0比125.0±7.5,t=18.55,P<0.05);T24细胞株中PDGFA-L和PDGFA-S组迁移数明显高于对照组(733±Objective To explore the effects of different transcriptional variants of platelet-derived growth factor A(PDGFA),PDGFA-L and PDGFA-S,on the proliferation and migration of bladder cancer cells.Methods The exome sequencing data in the cancer genome atlas(TCGA)were analyzed by bioinformatics methods to screen out abnormal alternative splicing events of PDGFA in bladder cancer.PDGFA-L and PDGFA-S were overexpressed in bladder cancer cell lines(EJ and T24).The experimental groups included EJ and T24 cell lines control group,EJ(EJ-PDGFA-L group and EJ-PDGFA-S group)and T24 cell lines(T24-PDGFA-L group and T24-PDGFA-S group)stably overexpressing PDGFA.Cell counting kit-8(CCK-8)assay,plate clone formation assay and Transwell assay were used to observe the effect of PDGFA on the proliferation and migration of bladder cancer.Independent sample t test was used for comparison between groups.Results Through bioinformatics analysis of the exon sequencing data of PDGFA gene in each tumor,abnormal alternative splicing of PDGFA was found in a variety of cancers,including bladder cancer.As compared with normal tissues,the expression of PDGFA-L in tumor tissues was higher than that of PDGFA-S(P<0.05).The results of CCK-8 showed that as compared with the control group,the proliferation ability of PDGFA overexpression group was significantly increasedthe optical density of EJ cells in PDGFA-L and PDGFA-S groups was significantly higher than that in the control group[(1.958±0.081 vs.0.982±0.357,t=12.765,P<0.05)and(1.324±0.476 vs.0.982±0.357,t=17.977,P<0.05)].In T24 cell lines,the optical density of PDGFA-L and PDGFA-S groups was significantly higher than that of the control group[(1.619±0.053 vs.0.821±0.029,t=17.61,P<0.05)and(1.219±0.073 vs.0.821±0.029,t=16.32,P<0.05)].The results of colony formation assay showed that the proliferation ability of PDGFA overexpression group was significantly higher than that of control group the colony number of EJ cell line in PDGFA-L and PDGFA-S group colony number was significantly greater

关 键 词：膀胱癌 选择性剪接 血小板衍生生长因子A 转录变体 

分 类 号：R737.14[医药卫生—肿瘤]