血小板促进肺腺癌细胞H460生成血管拟态的作用与机制研究  

Study on the role and mechanism of blood platelet promoting vasculogenic mimicry in lung adenocarcinoma cell H460

作　　者：陈诗雨 吴晓红 叶明君 陈敏 吴阳培 陈淑君 李白坤[1,2] CHEN Shiyu;WU Xiaohong;YE Mingjun;CHEN Min;WU Yangpei;CHEN Shujun;LI Baikun(School of Traditional Chinese Medicine,Anhui University of Traditional Chinese Medicine,Hefei,Anhui 230012,China;Research And Technology Center,Anhui University of Traditional Chinese Medicine,Hefei,Anhui 230012,China;School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei,Anhui 230012,China)

机构地区：[1]安徽中医药大学中医学院,安徽合肥230012 [2]安徽中医药大学科研实验中心,安徽合肥230012 [3]安徽中医药大学药学院,安徽合肥230012

出　　处：《临床肺科杂志》2024年第6期818-822,共5页Journal of Clinical Pulmonary Medicine

基　　金：安徽省自然科学基金项目(No.2208085MH279)。

摘　　要：目的 探讨血小板(Platelet, PLT)促进肺腺癌细胞H460生成血管拟态(vasculogenic mimicry, VM)的作用。方法 设立H460、PLT+H460,PLT+H460+Bev(贝伐单抗)3个组,采用三维细胞培养检测PLT对H460的VM生成作用,CCK8实验、划痕实验进一步检测细胞增殖和细胞迁移力的变化,ELISA法检测上清中的VEGF含量,Western Blot检测VM相关蛋白(VE-Cadherin、EphA2)的表达水平。结果 相较于H460组,PLT+H460组管道结构明显增多,增殖率增高,迁移力增强,PLT+H460+Bev组的管道结构、增殖率和迁移力均低于PLT+H460组。PLT+H460组的VEGF、VE-Cadherin、EphA2表达量均较H460组多,而PLT+H460+Bev组上述蛋白表达水平则低于PLT+H460组。结论 PLT可能促进H460细胞形成VM。Objective To explore the role of blood platelet(PLT)in vasculogenic mimicry(VM)formation of lung adenocarcinoma cells H460.Methods It set up three groups:the H460 group,the PLT+H460 group and the PLT+H460+Bev(Bevacizumab)group.It used three-dimensional cell culture to detect the VM generation effect of PLT on H460.CCK8 experiment and scratch experiment were further used to detect the proliferation and migration ability of H460.ELISA assay was used to detect VEGF content in the supernatant,and western blot was used to detect the expression level of VM related proteins(VE-Cadherin,EphA2).Results Compared with the H460 group,the PLT+H460 group showed significant increase in pipeline structure,proliferation rate,and migration ability.The pipeline structure,proliferation rate,and migration ability of the PLT+H460+Bev group were lower than those of the PLT+H460 group.Similarly,the expression levels of VEGF,VE-Cadherin,and EphA2 in the PLT+H460 group were higher than those in the H460 group,while the expression levels of these proteins in the PLT+H460+Bev group were lower than those in the PLT+H460 group.Conclusion PLT can promote the formation of VM in H460 cells.

关 键 词：血管生成拟态 血小板 肺腺癌细胞 实验研究 

分 类 号：R734.2[医药卫生—肿瘤]