推拿对睡眠剥夺小鼠基底前脑腺苷及A_(2A)受体的调节作用  

Regulation of Tuina on Sleep Deprivation via Adenosine and A_(2A)Receptor within Basal Forebrain in Mice

作　　者：孙冰心 袁露月 马妮妮 彭皑诗 胡毓诗[2] 康良 Sun Bingxin;Yuan Luyue;Ma Nini;Peng Aishi;Hu Yushi;Kang Liang(Physical Education Institute,Zhengzhou University of Science and Technology,Zhengzhou,450064,China;College of Sports Medicine and Health,Chengdu Sport University,Chengdu,610041,China;Rehabilitation Department,Zheng'an County Traditional Chinese Medicine Hospital,Zunyi,563400,China;Institute of Sports Medicine and Health,Chengdu Sport University,Chengdu,610041,China)

机构地区：[1]郑州科技学院体育学院,河南郑州450064 [2]成都体育学院运动医学与健康学院,四川成都610041 [3]遵义市正安县中医院康复科,贵州遵义563400 [4]成都体育学院运动医学与健康研究所,四川成都610041

出　　处：《成都中医药大学学报》2024年第3期18-22,35,共6页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家重点研发计划“科技冬奥”专项课题(2019YFF0301704);国家自然科学基金项目(81704190,82074576);四川省中医药管理局课题(2021MS444);四川省专家工作站资助课题(A2020-SCZJJCB02,A2020-SCZJZHC02)。

摘　　要：目的:研究推拿对睡眠剥夺小鼠基底前脑腺苷、A_(2A)受体的调节作用。方法:C57BL/6J小鼠随机分为空白组、模型组和推拿组,每组8只。经4 d睡眠剥夺并予6 d推拿治疗后,观察小鼠整体状态和日常活动量;以电生理记录脑电/肌电信号,分析小鼠觉醒、非快速眼动及快速眼动睡眠时间及基底前脑神经活动;ELISA法检测基底前脑腺苷水平;光纤记录法观察基底前脑星形胶质细胞释放腺苷情况;Western Blot法检测基底前脑腺苷A_(2A)受体蛋白表达。结果:①推拿干预后,睡眠剥夺小鼠毛发色泽和精神状态有所恢复;推拿组第4-6天日常活动量显著高于模型组(P<0.01);且第6天与空白组差异无统计学意义(P>0.05)。②推拿干预后,睡眠剥夺小鼠非快速眼期缩短(P<0.01),觉醒期增加(P<0.01);基底前脑δ、θ波活动降低。③推拿干预后,睡眠剥夺小鼠基底前脑腺苷水平降低(P<0.01);星形胶质细胞腺苷释放减少;A_(2A)受体蛋白表达下降(P<0.05)。结论:(1)推拿可增加睡眠剥夺小鼠日常活动,降低基底前脑δ、θ波活动,改善睡眠剥夺引发的嗜睡现象。(2)推拿可降低睡眠剥夺小鼠基底前脑腺苷水平及其受体A_(2A)蛋白表达,减少基底前脑星形胶质细胞腺苷释放,参与改善睡眠剥夺后觉醒过程。Objective:To reveal the effect of Tuina on sleep deprivation and the influence of adenosine and A_(2A)receptor within basal forebrain.Methods:Sixty-six C57BL/6J mice were randomly divided into blank group,model group and Tuina group.After the sleep deprivation model was prepared by sleep deprivation device for 4 days and Tuina treatment for 6 days,the mice were observed for general conditions and daily activities.Electroencephalogram and electromyogram signals were recorded through ethology.The mice were analyzed for Wake,non-rapid eye movement(NREM),rapid eye movement(REM)sleep phases and basal forebrain neural activity.ELISA was used to calculate the adenosine content in basal forebrain.The fiber photometry was used to observe the dynamic signals of astrocyte-released adenosine in basal forebrain.Western Blot was utilized to measure the protein expression of adenosine A_(2A)receptor in basal forebrain.Results:①Tuina mostly improve the behavioral outlook and daily activity in sleep-deprived mice.First,the hair color and mental phenotype of the sleep-deprived mice were recovered after Tuina treatment.Second,the daily activity of the Tuina group was significantly higher than that of the model group on the 4th-6th day(P<0.01),and the difference between the activity on the 6th day and that of the blank group was not statistically significant(P>0.05).②Tuina probably improve the somnolence phenomenon induced by sleep deprivation and the neural activity of basal forebrain.The NREM phase decreased significantly after Tuina treatment(P<0.01)while the Wake phase was significantly increased(P<0.01),and theδandθwave activities in basal forebrain was decreased.③Tuina possibly regulate adenosine and A_(2A)receptor of basal forebrain in sleep-deprived mice.The content of adenosine in sleep-deprived mice basal forebrain decreased significantly after Tuina(P<0.01).Meanwhile,the astrocyte-released adenosine and the protein expression of A_(2A)receptor were both decreased after Tuina-therapy(P<0.05).Conclusion:(1)Tuina inc

关 键 词：推拿 睡眠剥夺 基底前脑 腺苷 A_(2A)受体 

分 类 号：R224.1[医药卫生—中医基础理论] R338[医药卫生—中医学]