刺梨果实不同发育时期转录组分析及RACE法克隆CNR基因  

Transcriptome Analysis and Cloning of CNR Gene by RACE Method of Rosa roxburghii Tratt.Fruit at Different Developmental Stages

作　　者：陈宏宇 杨烨 于莹 田媛 CHEN Hongyu;YANG Ye;YU Ying;TIAN Yuan(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]贵州中医药大学基础医学院,贵阳550025

出　　处：《种子》2024年第5期7-15,共9页Seed

基　　金：国家自然科学基金项目(82360853);贵州中医药大学学术新苗(贵科合学术新苗[2023]-18号);贵州中医药大学博士启动基金([2020]19号)。

摘　　要：为研究刺梨果实不同发育期的差异表达基因,对不同发育期果实进行转录组测序。结果表明,共组装了98752个单基因,其中有64003个基因可被数据库注释。Ⅰ期vsⅡ期有上调基因1842个,下调基因1159个;Ⅰ期vsⅢ期有上调基因911个,下调基因538个。刺梨果实不同时期的基因表达差异较大,这些基因表达差异为探索刺梨果实发育机制提供参考。CNR基因即细胞数量调控因子,是一个植物的细胞数量负调控因子,可能调控果实发育。本研究利用RACE技术克隆得到一个刺梨CNR基因的cDNA全长序列,并构建真核表达载体,为后续深入研究基因功能提供基础。In order to study the differentially expressed genes of Rosa roxburghii Tratt.fruits at different development stages,transcriptome sequencing was performed on the fruits at different development stages.The results showed that a total of 98752 single genes were assembled,of which 64003 genes could be annotated by the database.There were 1842 up-regulated genes and 1159 down-regulated genes in stageⅠvs stageⅡ.There were 911 up-regulated genes and 538 down-regulated genes in stageⅠvs stageⅢ.The differences in gene expression in different periods of Rosa roxburghii Tratt.fruit were big,which provided a reference for exploring the mechanism of Rosa roxburghii Tratt.fruit development.CNR gene,cell number regulator,was a negative regulator of plant cell number and could regulate fruit development.In this study,a full-length cDNA sequence of CNR gene was cloned using RACE technology,and the eukaryotic expression vector was constructed,which provided the basis for further study of gene function.

关 键 词：刺梨 果实 转录组 细胞数量调控因子 基因 

分 类 号：Q781[生物学—分子生物学]