金针菇免疫调节蛋白FIP-fve在毕赤酵母中的表达研究  

Expression of immunomodulatory protein FIP-fve from Flammulina velutipes in Pichia pastoris

作　　者：杜婉嘉 陈香利 毛雪 马银鹏[1] 孔祥辉[1] 林火松 DU Wanjia;CHEN Xiangli;MAO Xue;MA Yinpeng;KONG Xianghui;LIN Huosong(Institute of Microbiology Heilongjiang Academy of Sciences,Harbin 150010,China;Longquan Bureau of Agriculture and rural Cash crop service station Service Station,Longquan 323700,China)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]龙泉市农业农村局经济作物服务站,浙江龙泉323700

出　　处：《食药用菌》2024年第3期176-181,共6页Edible and Medicinal Mushrooms

基　　金：黑龙江省科学院院长基金项目(YZ2023sw01)。

摘　　要：金针菇免疫调节蛋白FIP-fve是隶属于真菌免疫调节蛋白家族的小分子功能蛋白质,在生物医学研究和功能食品应用中有重要价值,但天然蛋白产量较低。本文构建了真核表达载体pPIC9K-FIP-fve后转入毕赤酵母GS115。结果:甲醇诱导获得高效分泌表达的可溶性蛋白,表达率40%以上,经纯化,蛋白产率为121.6 mg/L。本研究为FIP-fve重组蛋白应用奠定了基础。Flammulina velutipes immunomodulatory protein fve(FIP-fve),a member of the fungal immunomodulatory protein family,is a small molecular functional protein with significant biomedical research and functional food applications.However,the natural protein yield is low.In this study,a eukaryotic expression vector pPIC9K-FIP-fve was constructed based on pUC19-FIP-fve,which was transferred into Pichia pastoris GS115.The result showed that methanol induction led to efficient secretion of soluble protein,with an expression level of over 40%.After purification,the protein yield was 121.6 mg/L.This research lays the foundation for the application of recombinant FIP-fve protein.

关 键 词：金针菇免疫调节蛋白 真核重组载体构建与表达 纯化 

分 类 号：S646[农业科学—蔬菜学]