GLUT-1在三阴性乳腺癌中的表达及其与临床病理特征和预后的关系研究  

Expression of glucose transporter 1 in triple-negative breast cancer and its relationship with the clinicopathological charac-teristics and prognosis

作　　者：薛丽 陆凯 XUE Li;LU Kai(Department of Breast Disease,the First Hospital of Jiaxing,Jiaxing 314000,China)

机构地区：[1]嘉兴市第一医院乳腺病科,314000

出　　处：《浙江医学》2024年第10期1074-1078,1082,共6页Zhejiang Medical Journal

基　　金：嘉兴市科技计划项目(2020AD30066)。

摘　　要：目的分析三阴性乳腺癌(TNBC)中葡萄糖转运蛋白1(GLUT-1)的表达水平,并探讨其与患者临床病理特征和预后的关系。方法回顾性选取2019年1月至2021年1月在嘉兴市第一医院行乳腺癌根治术的TNBC患者79例(TNBC组),另择同期本院乳腺良性疾病(包括乳腺炎、乳腺纤维瘤等)患者40例为对照(对照组)。留取TNBC组患者的肿瘤组织与癌旁组织,对照组患者乳腺活检组织。采用qRT-PCR法检测组织标本GLUT-1 mRNA表达情况。收集TNBC组患者的临床病理资料,术后每3个月随访1次,随访时间截至2023年12月,其中随访1年时出现死亡、肿瘤复发进展、出现严重并发症(恶病质)等定义为预后不良。比较TNBC组肿瘤组织、癌旁组织与对照组乳腺活检组织GLUT-1 mRNA表达水平;比较不同临床病理特征的TNBC组患者肿瘤组织GLUT-1 mRNA表达水平;分析不同临床病理特征对TNBC患者术后1年预后不良的预测效能、TNBC患者死亡的危险因素及不同GLUT-1 mRNA表达水平TNBC患者生存情况。结果TNBC组肿瘤组织GLUT-1 mRNA表达水平高于癌旁组织、对照组乳腺活检组织(均P<0.05),而TNBC组癌旁组织与对照组乳腺活检组织GLUT-1 mRNA表达水平比较差异无统计学意义(P>0.05)。低分化、肿瘤直径≥5 cm、Ki-67指数≥70%、有神经脉管浸润、有淋巴结转移、有远处转移及TNM分期Ⅲ期的TNBC组患者肿瘤组织GLUT-1 mRNA表达水平分别高于中-高分化、肿瘤直径<5 cm、Ki-67指数<70%、无神经脉管浸润、无淋巴结转移、无远处转移及TNM分期Ⅰ~Ⅱ期的患者(均P<0.05)。肿瘤组织GLUT-1 mRNA表达水平预测TNBC患者术后1年预后不良的灵敏度、特异度及AUC分别为0.845、0.812、0.842,最佳截断值为0.83,有较高的预测效能(P<0.05)。多因素Cox回归分析显示,GLUT-1 mRNA表达水平≥0.83是TNBC患者死亡的独立危险因素(P<0.05)。肿瘤组织GLUT-1 mRNA表达水平≥0.83的TNBC患者生存期低于<0.83的患者[(27Objective To analyze the expression level of glucose transporter 1(GLUT-1)in triple-negative breast cancer(TNBC)and explore its relationship with the clinicopathological characteristics and prognosis of patients.Methods Seventy-nine patients with TNBC(TNBC group)who underwent radical surgery in the First Hospital of Jiaxing from January 2019 to January 2021 were selected,and another 40 patients with benign breast diseases(including mastitis,mammary fibroma,etc.)admitted during the same period were enrolled as the control group.Tumor tissues and adjacent tissues of TNBC group and breast biopsy tissues of the control group were collected.The qRT-PCR method was used to detect the expression of GLUT-1 mRNA in tissue specimens.The clinical and pathological data of patients in the TNBC group were collected and followed up every 3 months after surgery until December 2023.Death,tumor recurrence and progression,severe complications(cachexia)and so on at one year of follow-up were defined as poor prognosis.The expression levels of GLUT-1 mRNA in tumor and adjacent tissues of TNBC group and breast biopsy tissues of the control group were compared,so were the expression levels of GLUT-1 mRNA in tumor tissues of patients in TNBC group with different clinicopathological characteristics to analyze the performance of different clinicopathological characteristics in predicting poor prognosis of TNBC patients one year after surgery,the risk factors for death of TNBC patients,and the survival of TNBC patients with different GLUT-1 mRNA expression levels.Results The expression level of GLUT-1 mRNA in the tumor tissue of the TNBC group was higher than that in the adjacent tissue and that of the breast biopsy tissue of the control group(both P<0.05).However,there was no difference in the expression level of GLUT-1 mRNA between the adjacent tissue of TNBC group and the breast biopsy tissue of the control group(P>0.05).GLUT-1 mRNA expression level in tumor tissue of TNBC patients with poor differentiation,tumor diameter≥5 cm,Ki-67 ind

关 键 词：三阴性乳腺癌 葡萄糖转运蛋白1 临床病理特征 预后 临床价值 

分 类 号：R737.9[医药卫生—肿瘤]