油莎草三部位提取物抗抑郁作用筛选及成分分析  

Antidepressant Effects Screening of Three Parts of Cyperus esculentus Extracts and Its Component Analysis

作　　者：张文军 敬思群 杨金梅 朱建华[1] 张俊艳[1] 赖胤龙 陈洁 ZHANG Wen-jun;JING Si-qun;YANG Jin-mei;ZHU Jian-hua;ZHANG Jun-yan;LAI Yin-long;CHEN Jie(Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region/Shaoguan University,Shaoguan 512005,China;College of Life and Geographic Sciences,Kashi University,Kashi 844006,China)

机构地区：[1]广东省粤北食药资源利用与保护重点实验室/韶关学院,广东韶关512005 [2]喀什大学生命与地理科学学院,新疆喀什844006

出　　处：《中国食物与营养》2024年第5期60-67,共8页Food and Nutrition in China

基　　金：广东省教育厅广东省普通高校重点研究项目(项目编号:2019KZDXM053);2021年度广东省自然科学基金面上项目(项目编号:2021A1515011262);国家自然科学基金面上项目(项目编号:31770385)。

摘　　要：目的:利用皮质酮诱导的果蝇抑郁症模型,分析油莎草三部位提取物的抗抑郁作用,同时对抗抑郁作用最强的油莎草须根挥发油的成分进行分析,以期为油莎草抗抑郁作用的深入研究提供理论基础。方法:以DPPH·清除率、ABTS+·清除率及总抗氧化能力评价油莎草三部位提取物的体外抗氧化能力;将雄性果蝇随机分为对照组、模型组、氟西汀组和油莎草全株三部位提取物高剂量组、低剂量组,以SOD、MDA、5-HA为考察指标,分析油莎草全株三部位提取物对果蝇抑郁模型的影响。采用顶空固相微萃取-气相色谱质谱技术(HS-SPME/GC-MS)分析了须根挥发油成分。结果:油莎草三部位提取物以油莎草须根挥发油的体外抗氧化性能力最强。三部位提取物均可提高皮质酮诱导果蝇抑郁模型中的SOD及5-HT含量,降低MDA,抗抑郁最强的部位为油莎草须根挥发油。酮类、醛类、醇类、酯类、酚类、酸类是油莎草须根挥发油的主要组成。结论:油莎草三部位提取物均具有抗抑郁作用,可能与含有的挥发性成分尤其是酮类有关。【Objective】The antidepressant effects of extracts from three parts of Cyperus esculentus by using Corticosterone-Induced Drosophila Depression Model,and the components of the volatile oil of rhizome root of Cyperus esculentus which has the strongest antidepression effect were analyzed.【Method】The mentioned above could provide theoretical basis for further research on the antidepressant ef⁃fects of Cyperus esculentus.DPPH·clearance,ABTS+·clearance and total antioxidant capacity were used to evaluate the antioxidant capac⁃ity in vitro.Moreover,male fruit flies were randomly divided into control group,model group,fluoxetine group,high-dose group and lowdose group,and SOD,MDA and 5-HT were used as indicators to investigate the antidepressant effect of extracts from all parts of the whole plant of Cyperus esculentus.The components of volatile oil in fibrous root were analyzed by HS-SPME/GC-MS.【Result】The most antidepressant parts of the extracts of Cyperus esculentus were the alcohol extracts of Cyperus esculentus leaves and the essential oil of Cyperus esculentus fibrous roots in vitro.All the extracts of the three parts could increase SOD and 5-HT content and decrease MDA content in Drosophila Depression Model induced by corticosterone.The strongest anti-depression parts were the volatile oil of rhizome root of Cyperus esculentus.【Conclusion】The extracts from three parts of Cyperus esculentus have anti-depressant effects,which may be related to the volatile components,especially ketones.

关 键 词：油莎草三部位 抗氧化能力 果蝇抑郁症模型 固相微萃取与气质联用(HS-SPME/GC-MS) 

分 类 号：R285[医药卫生—中药学]