硒对六个青稞品种种子萌发及吸收转运的影响  

Effects of Selenium on Germination and Selenium Uptake and Transportation in Six Varieties of Highland Barley

作　　者：高雪 李安庆 冉黄丹妮 卓玛 孙媛媛 GAO Xue;LI Anqing;RAN Huangdanni;ZHUO Ma;SUN Yuanyuan(Institute of Resources and Environment,Xizang Academy of Agricultural and Animal Husbandry Sciences/State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement,Lhasa,Xizang 850002;School of Life Sciences,Guizhou Normal University/Key Laboratory of Plant Physiology and Developmental Regulation of Guizhou province,Guiyang,Guizhou 550001)

机构地区：[1]西藏自治区农牧科学院农业资源与环境研究所/省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,西藏拉萨850002 [2]贵州师范大学生命科学学院/贵州省植物生理与发育调控重点实验室,贵州贵阳550001

出　　处：《核农学报》2024年第7期1385-1397,共13页Journal of Nuclear Agricultural Sciences

基　　金：西藏自治区科技厅重点研发计划项目(XZ202001ZY0042N)。

摘　　要：为探明不同价态硒对青稞(Hordeum vulgare L.var.nudum Hook.f.)种子萌发、生长及其吸收转运硒的影响,本研究以6个青稞品种为研究对象,通过种子萌发试验,研究不同价态(Se^(6+)和Se^(4+))和不同浓度水平(0~5.0 mg·L^(-1)和0~40 mg·L^(-1))对青稞种子萌发指标的影响;利用加权隶属函数法对不同价态和不同浓度硒水平下青稞种子的萌发能力进行综合评价;通过水培试验研究青稞对硒的吸收转运。结果表明,随着硒浓度的升高,6个青稞品种种子的发芽势、发芽率、发芽指数和活力指数呈先增加后减小趋势。加权隶属函数值(D值)结果表明,Se^(6+)和Se^(4+)浓度在0~1.0 mg·L^(-1)范围内可促进青稞种子萌发。与Se^(6+)对青稞种子生长的影响不同,当Se^(4+)浓度大于1.0 mg·L^(-1)时,Se^(4+)对胚芽和胚根的抑制作用更为明显。Se^(6+)处理青稞地上部分硒含量高于Se^(4+)处理,6个青稞品种对Se^(6+)的吸收量约为对Se^(4+)吸收量的1.39~4.21倍,以山青9号地上部分硒吸收量最高。6个青稞品种对Se^(6+)的转运系数在0.671~3.342之间;除藏青3000对Se^(6+)的转运系数小于1外,其他5个青稞品种对Se^(6+)的转运系数在1.503~3.342之间,均大于1,而对Se^(4+)的转运系数在0.192~0.319之间,均小于1。本研究结果可为西藏青稞硒生物强化提供理论依据。This study aimed to investigate the impact of various selenium valence states on seed germination,growth,and selenium uptake and translocation in six selected varieties of highland barley(Hordeum vulgare L.var.nudum Hook.f.).The effects of different selenium valence states(Se^(6+)and Se^(4+))at varying concentrations(0-5.0 mg·L^(-1) and 0-40 mg·L^(-1))on multiple indicators of highland barley seed germination were investigated through a series of experimental trials.The germination ability of highland barley seed was comprehensively evaluated using the weighted membership function method under different valence states and concentrations.Hydroponic experiments were conducted to investigate the selenium absorption and transportation of six varieties of highland barley.The results indicate that,as the selenium concentration increases,the germination potential,germination rate,germination index,and vitality index of highland barely exhibit an initial increased and followed by a decrease trend.The analysis of the weighted membership function values(D-values)indicated that the germination rate of highland barley seeds could be promoted when concentrations of Se^(6+)and Se^(4+)ranging from 0 to 1.0 mg·L^(-1).In contrast to the impact of Se^(6+)on barley seed growth,the inhibitory effect of Se^(4+)on germ and radicle became more pronounced when its concentration exceeded 1.0 mg·L^(-1).The selenium content in the shoots of highland barely treated with Se^(6+)exhibited higher levels compared to those treated with Se^(4+).The absorption amount of Se^(6+)by the six varieties of highland barely were approximately 1.39 to 4.21 times of those of Se^(4+),with Shanqing No.9 demonstrating the highest selenium uptake in shoots.Among the six varieties of highland barley,the transport coefficients for Se^(6+)ranged from 0.671 to 3.342.Except for Zangqing 3000,which had a transport coefficient less than 1 for Se^(6+),the other five varieties exhibit transport coefficients ranged from 1.503 to 3.342.As for Se^(4+),the transport coe

关 键 词：青稞 硒价态 种子萌发 吸收 转运 

分 类 号：S512.3[农业科学—作物学]