多维度综合评价瞬时高温灭菌对丹参质量的影响  

Multi-Dimensional Comprehensive Evaluation of the Impact of Instantaneous High-Temperature Sterilization on the Quality of Salvia Miltiorrhiza

作　　者：庄新慧 焦连庆[1] 于敏[1] 田齐聪 张凤清[2] ZHUANG Xin-hui;JIAO Lian-qing;YU Min;TIAN Qi-cong;ZHANG Feng-qing(Jinlin Provincial Academy of Traditional Chinese Medicine,Changchun 130012,China;School of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130012,China)

机构地区：[1]吉林省中医药科学院,吉林长春130012 [2]长春工业大学化学与生命科学学院,吉林长春130012

出　　处：《化学试剂》2024年第6期9-16,共8页Chemical Reagents

基　　金：吉林省发展和改革委员会项目(2020C032-6)。

摘　　要：为考察瞬时高温灭菌对丹参质量的影响,采用HPLC法测定10批灭菌前后丹参中8种化学成分含量,DPPH·法测定丹参灭菌前后抗氧化活性,MTT法测定灭菌前后丹参大鼠肝星状细胞(HSC-T6)存活率。建立HPLC指纹图谱,液质联用(LC-MS)法确定共有峰结构,采用聚类分析和正交偏最小二乘法判别分析(OPLS-DA)预测不同丹参差异性质量标志物;通过丹参与过量DPPH反应后的HPLC峰面积变化,筛选丹参抗氧化成分;采用偏最小二乘法回归(PLS-DA)分析法,得出共有峰与DPPH·抗氧化活性间的谱效关系,结合单体化合物抗氧化活性大小,最终确定丹参抗氧化活性质量标志物。灭菌前后8种化学成分含量、DPPH·清除率及细胞存活率经t检验均无显著性差异(P>0.05);灭菌前后指纹图谱相似度均大于0.9,同一批次样品灭菌前后聚为一类,瞬时高温灭菌对丹参质量未产生影响;紫草酸甲酯、丹酚酸B、丹参酸甲酯、丹参酮Ⅰ、隐丹参酮、丹酚酸F、丹参酮ⅡA、二氢丹参酮Ⅰ可作为丹参质量差异标志物;迷迭香酸、丹酚酸B、紫草酸、丹酚酸F可作为抗氧化活性的质量标志物。To investigate the impact of instantaneous high-temperature sterilization on the quality of Salvia miltiorrhiza,the content of eight chemical components in Salvia miltiorrhiza before and after sterilization was determined using HPLC,the antioxidant activity of Salvia miltiorrhiza before and after sterilization was measured using the DPPH·method,and the cell viability of rat hepatic stellate cells(HSC-T6)before and after sterilization was measured using the MTT method.Set up HPLC fingerprints,the shared peak structures were determined using liquid chromatography-mass spectrometry(LC-MS),differential quality markers for different batches of Salvia miltiorrhiza were obtained through cluster analysis and orthogonal partial least squares discriminant analysis(OPLS-DA).The antioxidant components of Salvia miltiorrhiza were screened by HPLC peak area changes after reaction with excess DPPH.Partial least square regression(PLS-DA)analysis method was used to obtain the spectral effect relationship between the common peak and DPPH·antioxidant activity,combined with the antioxidant activity of monomer compounds,and finally determine the antioxidant activity quality markers of Salvia miltiorrhiza.The contents of 8 chemical components,DPPH·clearance rate and cell survival rate were not significantly different by t test before and after sterilization(P>0.05).The similarity of fingerprints before and after sterilization was greater than 0.9,the same batch of samples clustered into one class before and after sterilization,instantaneous high temperature sterilization had no effect on the quality of Salvia miltiorrhiza.Methyl vioxalate,salvianolic acid B,methyl salvianolic acid,tanshinoneⅠ,cryptotanshinone,salvianolic acid F,tanshinoneⅡA and dihydrotanshinoneⅠcould be used as markers of quality difference of salvianone.Rosmarinic acid,salvianolic acid B,purple oxalic acid,salvianolic acid F can be used as quality markers of antioxidant activity.

关 键 词：丹参 瞬时高温灭菌 DPPH·抗氧化活性 质量标志物 质量评价 

分 类 号：R284[医药卫生—中药学]