Er,Cr:YSGG激光治疗对高糖环境下微螺钉周围炎症及对RANK/RANKL信号通路的影响  

Effects of Er,Cr:YSGG laser therapy on inflammation around microscrews and RANK/RANKL signaling pathway in hyperglycemic environment

作　　者：刘阳 牛家慧[1] LIUYang;NIU Jia-hui(Department of Stomatology,The Second Hospital of Shijiazhuang,Shijiazhuang 050000,China)

机构地区：[1]河北省石家庄市第二医院口腔科,石家庄050000

出　　处：《口腔颌面修复学杂志》2024年第3期170-174,共5页Chinese Journal of Prosthodontics

基　　金：石家庄市科学技术研究与发展计划项目(项目编号:211461273)。

摘　　要：目的:探讨铒铬铱钪镓石榴石(Er,Cr:YSGG)激光治疗对高糖环境下微螺钉周围炎症治疗的疗效,并分析与RANK/RANKL信号通路的关系。方法:20只正常健康雄性新西兰兔随机分为A组(微螺钉周健康组)、B组(微螺钉周围炎症组)、C组(微螺钉周围炎症激光治疗组),皮下注射2%四氧嘧啶建立糖尿病模型,下颌双侧无牙区植入微螺钉种植体,3个月后,A组进行菌斑控制,B组、C组用丝线拴结于微螺钉种植体颈部基台处引入炎症,C组予以Er,Cr:YSGG激光治疗。记录菌斑指数、探诊深度、牙龈指数和龈沟液量;ELISA检测龈沟液炎性细胞白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平;CBCT检查微螺钉种植体周围炎周围骨吸收状态;Western blot检测牙龈组织中核因子κB(NF-κB)/NF-κB受体性活化因子(RANK)/NF-κB受体活化因子配体(RANKL)通路相关蛋白表达。结果:CBCT检查显示与A组比较,B组微螺钉种植体周围牙槽骨有明显的炎性吸收,微螺钉种植体-骨界面愈合差,骨附着不足;与B组比较,C组一侧牙槽骨有少量新生纤维成骨。与A组比较,B组PI、PD、GI水平较高,龈沟液量较多,龈沟液中IL-1β、TNF-α和IL-6水平较高,牙龈中NF-κBp65、RANK、RANKL蛋白表达水平较高(P<0.05);与B组比较,C组PI、PD、GI水平较低,龈沟液量较少,龈沟液中IL-1β、TNF-α和IL-6水平较低,牙龈中NF-κBp65、RANK、RANKL蛋白表达水平较低(P<0.05)。结论:Er,Cr:YSGG激光治疗糖尿病兔颌骨微螺钉种植体周围炎的疗效显著,且在NF-κB/RANK/RANKL通路表达调控上可能发挥一定作用。Objective:To investigate the therapeutic effect of erbium-chromium-Irdium-scandium gallium garnet(Er,Cr:YSGG)laser therapy on inflammation around microscrews in high glucose environment,and to analyze the relationship with RANK/RANKL signaling pathway.Methods:Twenty normal healthy male New Zealand rabbits were randomly divided into group A(healthy group around microscrews),group B(inflammation group around microscrews)and group C(laser treatment group around microscrews).The diabetes model was established by subcutaneous injection of 2%alloxouracine,and microscrew implants were implanted in mandibular edenceless areas on both sides.Group B and group C were treated with Er,Cr:YSGG laser for inflammation induced at the neck abutment of microscrew implant.The plaque index,probing depth,gingival index and gingival crevicular fluid volume were recorded.The levels of interleukin-1β(IL-1β),tumor necrosis factor(TNF-α)and interleukin-6(IL-6)in gingival crevicular fluid inflammatory cells were detected by ELISA.CBCT was used to examine the bone resorption status around microscrew implant periinflammation.Western blot analysis of nuclear factorκB(NF-κB)/NF-κB receptor activator(RANK)/NF-κB receptor activator ligand(RANKL)path-related protein expression in gum tissue.Results:CBCT examination showed that compared with group A,the alveolar bone around the microscrew implant in group B had obvious inflammatory absorption,and the microscrew implant-bone interface healing was poor,and bone attachment was insufficient.Compared with group B,there was a small amount of new fiber osteogenesis in one alveolar bone of group C.Compared with group A,group B had higher levels of PI,PD and GI,more gingival crevicular fluid,higher levels of IL-1β,TNF-αand IL-6 in gingival crevicular fluid,and higher expression levels of NF-κBp65,RANK and RANKL in gingival fluid(P<0.05).Compared with group B,the levels of PI,PD and GI in group C were lower,the amount of gingival crevicular fluid was lower,the levels of IL-1β,TNF-αand IL-6 in gin

关 键 词：铒铬铱钪镓石榴石激光 糖尿病 微螺钉周围炎 核因子κB(NF-κB)/NF-κB受体性活化因子(RANK)/NF-κB受体活化因子配体(RANKL)通路 

分 类 号：R780.2[医药卫生—口腔医学]