光磁双模态成像显示大鼠脑组织间液引流路径及物质清除规律  

Fluorescence and MR dual-mode imaging for displaying drainage pathways of interstitial fluid and substance clearance pattern in rat brain

作　　者：高天姿 袁兰 汪洋 谈瀚博 魏子伊 王家钰 高亚娟[2] 刘东阳 崔诚 孙剑飞[4] 谢肇恒 韩鸿宾 GAO Tianzi;YUAN Lan;WANG Yang;TAN Hanbo;WEI Ziyi;WANG Jiayu;GAO Yajuan;LIU Dongyang;CUI Cheng;SUN Jianfei;XIE Zhaoheng;HAN Hongbin(Institute of Medical Technology,Peking University Health Science Center,Beijing 100083,China;Department of Radiology,Peking University Third Hospital,Beijing 100191,China;Drug Clinical Trial Center,Peking University Third Hospital,Beijing 100191,China;School of Biological Science&Medical Engineering,Southeast University,Nanjing 210096,China;Beijing Key Laboratory of Magnetic Resonance Imaging Devices and Technology,Beijing 100191,China;NMPA Key Laboratory for Evaluation of Medical Imaging Equipment and Technique,Beijing 100191,China)

机构地区：[1]北京大学医学部医学技术研究院,北京100083 [2]北京大学第三医院放射科,北京100191 [3]北京大学第三医院药物临床试验机构,北京100191 [4]东南大学生物科学与医学工程学院,江苏南京210096 [5]北京市磁共振成像设备与技术重点实验室,北京100191 [6]国家药品监督管理局医学成像设备质量评价重点实验室,北京100191

出　　处：《中国医学影像技术》2024年第5期705-711,共7页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金重大项目(62394312、62394314、62394310)、北京市自然科学基金重点研究专题(Z210007)。

摘　　要：目的采用荧光示踪成像和磁示踪成像观察大鼠脑组织间液(ISF)引流路径及物质清除规律。方法将33只雄性SD大鼠随机分为荧光示踪组(F组,n=18)和磁示踪成像组(MRI组,n=15),再细分为丘脑亚组、海马亚组和尾状核亚组。对F组大鼠注射伊文斯蓝并行心脏灌流,取脑组织制成冰冻切片,观察各亚组示踪剂引流路径;对MRI组大鼠分别于注射钆喷酸葡胺(Gd-DTPA)前、后行MR扫描,对比各亚组脑区ROI信号强度,计算单位信号比值并观察其变化趋势。结果F组大鼠丘脑、海马、尾状核IFS引流分别存在优势路径,其到达相邻脑区及全脑的时间存在均差异。MRI组注射Gd-DTPA后4 h内丘脑、海马、尾状核示踪剂清除方向性及速率有所不同,表现为丘脑及海马区示踪剂向同侧皮层及侧脑室方向引流、尾状核区示踪剂向皮层和中脑方向扩散,且相邻引流脑区示踪剂信号达峰时间存在差异。结论光磁双模态成像显示,大鼠丘脑、海马及尾状核ISF优势引流路径及其内小分子物质清除速率均存在差异。Objective To observe the drainage pathways of interstitial fluid(ISF)and substance clearance pattern in rat brain with fluorescence tracing imaging and treacer-based MRI.Methods Thirty-three male SD rats were randomly divided into fluorescence tracing group(F group,n=18)and treacer-based MRI group(MRI group,n=15),then further divided into thalamic,hippocampal and caudate nucleus subgroups,respectively.Evans blue was injected to rats in F group,and cardiac perfusion was performed after injection,then brain tissue was harvested,and frozen sections were made to observe the drainage pathways of IFS in different subgroups.MRI was performed on rats in MRI group before and after injection of gadolinium-diethylenetriamine pentaacetic acid(Gd-DTPA)to observe signal intensity in ROI of brain regions in different subgroups,the signal unit ratio was calculated,and the changing trend was explored.Results ISF in thalamus,hippocampus and caudate nucleus had different dominant drainage pathways,and the time of tracer reached to adjacent brain regions and whole brain in F group were different.In MRI group,within 4 h after injection of Gd-DTPA,there were differences in direction and clearance rate among tracer in thalamus,hippocampus and caudate nucleus,mainly manifesting as the tracer in thalamus and hippocampus drained to the ipsilateral cortex and lateral ventricle,while the tracer in the caudate nucleus diffused to the cortex and midbrain,and there were differences of the peak time of tracer signal among adjacent drainage brain regions.Conclusion Fluorescence and MR dual-mode imaging showed that there were differences in the dominant drainage pathways of IFS and clearance rates of small molecule substances among hypothalamus,hippocampus and caudate nucleus of rats.

关 键 词：大鼠 脑 荧光 磁共振成像 

分 类 号：R742[医药卫生—神经病学与精神病学] R445[医药卫生—临床医学]