生长期对金耳多糖结构特征及抗炎活性的影响  

Effects of Cultivation Time on Structural Characteristics and Anti-Inflammatory Activity of Polysaccharides from Naematelia aurantialba

作　　者：刘利平 刘艳芳[1] 孙浩雯 冯杰[1] 杨林雷 曹瑶 李荣春 张劲松[1] LIU Liping;LIU Yanfang;SUN Haowen;FENG Jie;YANG Linlei;CAO Yao;LI Rongchun;ZHANG Jingsong(Institute of Edible Fungi Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Yunnan Junshijie Biotechnology Co.,Ltd.,Kunming 651708,Yunnan,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403 [2]云南菌视界生物科技有限公司,云南昆明651708

出　　处：《食用菌学报》2024年第3期42-50,共9页Acta Edulis Fungi

基　　金：国家现代农业产业技术体系(CARS-20);云南(昆明)张劲松药用真菌专家工作站(YSZJGZZ-2022043);云南省张劲松专家工作站(202305AF150084)。

摘　　要：对生长期为25、35、45 d的金耳子实体(Naematelia aurantialba)多糖(分别编号为NAP-1、NAP-2、NAP-3)的得率、多糖含量、糖醛酸含量、分子量分布特征、单糖组成及体外抗炎活性进行比较。结果表明,NAP-3的得率(34.50%)最高,多糖与糖醛酸含量显著高于NAP-1。随着生长期的延长,子实体多糖的重均分子量(weight average molecular weight,M_(w))由1.859×10~6 g·mol^(-1)降低至1.347×10~6 g·mol^(-1)。单糖组成分析结果表明,子实体多糖是一种由木糖、甘露糖、葡萄糖醛酸以及少量葡萄糖组成的酸性杂多糖。不同生长期的金耳子实体多糖均能抑制脂多糖诱导引起的RAW264.7巨噬细胞NO释放与炎症因子分泌,与NAP-2、NAP-3相比,NAP-1抑制效果最强。通过Pearson相关性分析,发现金耳子实体多糖的体外抗炎活性与其M_(w)和甘露糖的摩尔百分比呈正相关,与木糖的摩尔百分比呈负相关。研究结果可为采收获得高抗炎活性的金耳子实体提供技术参数,对金耳健康产品的开发利用提供参考。Polysaccharides were extracted from fruiting bodies of Naematelia aurantialba at 25 d,35 d and 45 d of cultivation,and were numbered NAP-1,NAP-2 and NAP-3,respectively.NAP-1,NAP-2 and NAP-3 were then compared for yield,polysaccharide content,glucuronic acid content,molecular weight distribution,monosaccharide composition,and anti-inflammatory activity in vitro.The results showed that NAP-3 had the highest yield of 34.50%,and its polysaccharide and glucuronic acid contents were higher than those of NAP-1.As the growth time extended,the weight average molecular weight(M_w) of N.aurantialba polysaccharides(NAPs)decreased from 1.859×10~6 g·mol~(-1) to 1.347×10~6 g·mol~(-1).Monosaccharide composition analysis revealed that NAPs were acidic heteropolysaccharides composed of xylose,mannose,glucuronic acid(GlcA),and a small amount of glucose.All three NAPs inhibited NO release and inflammatory factor secretion in RAW264.7 macrophages induced by lipopolysaccharide(LPS).Compared with NAP-2 and NAP-3,the anti-inflammatory efficacy of NAP-1 was better.Pearson's correlation analysis showed that the in vitro anti-inflammatory activity of N.aurantialba was positively correlated with M_(w) and the molar percentage of mannose,but negatively correlated with the molar percentage of xylose.These findings provided a reference for harvesting N.aurantialba fruiting bodies with high anti-inflammatory activity,and the development of N.aurantialba healthcare products.

关 键 词：金耳 不同生长期 多糖 分子量 单糖组成 抗炎活性 

分 类 号：S567.34[农业科学—中草药栽培]