广热带睡莲的挥发态气味物质检测及优良芳香品种筛选  

Detecting volatile aroma substances and screening elite aromatic varieties in pantropical water lilies

作　　者：吴秋霞 易齐贤 陈曦 樊熙蓓 唐昕妍 王景云 殷铭轩 郑日如 陈胜洪 袁慧红 WU Qiuxia;YI Qixian;CHEN Xi;FAN Xibei;TANG Xinyan;WANG Jingyun;YIN Mingxuan;ZHENG Riru;CHEN Shenghong;YUAN Huihong(Zhejiang Humanity Landscape Co.,Ltd.,Hangzhou 310029,China;National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops/College of Horticulture&Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]浙江人文园林有限公司,杭州310029 [2]华中农业大学园艺林学学院/果蔬园艺作物种质创新与利用全国重点实验室,武汉430070

出　　处：《华中农业大学学报》2024年第3期230-239,共10页Journal of Huazhong Agricultural University

基　　金：华中农业大学横向科研项目(305/707122465)。

摘　　要：为检测广热带亚属睡莲挥发性成分,鉴定香气活性物质,筛选优良芳香品种,采用顶空固相微萃取结合气相色谱-质谱联用技术(HS-SPEM-GC-MS)对30个广热带睡莲亚属常见栽培品种进行挥发态物质检测。结果显示,30个睡莲品种共含有60种挥发态物质,含量从高到低排列依次是烷烃、烯烃、萜类、芳香族、酯类、醇类、醛类7类化合物。其中,烷烃、烯烃是含量较高的类别,平均占比分别达到42.43%和28.61%,但其无明显气味特征。进一步通过气味活性值(OAV)筛选到了19种气味活性物质,其中可被嗅觉感知的(OAV>1)的特征气味物质均为萜类化合物,包括α-紫罗兰酮、β-紫罗兰酮、反式-β-紫罗兰酮、金合欢烯,其中紫罗兰酮赋予睡莲木质香、甜香,金合欢烯赋予睡莲苹果香及典型花香。结果表明,以气味活性值为标准筛选出的‘蓝鸟’‘卡拉阳光’‘黄金国’‘鲁比’‘粉钻’5个睡莲品种是园林应用和产品加工的潜在优良品种。The headspace solid-phase microextraction combined with gas chromatography-mass spectrometry(HS-SPEM-GC-MS)technology was used to detect volatile substances in 30 common cultivated varieties of pantropical water lilies to detect the volatile components,identify the aroma active substances,and screen the excellent aromatic varieties of water lilies.The results showed that a total of 60 volatile compounds were identified in the 30 varieties of pantropical water lilies,with seven compounds including alkanes,alkenes,terpenoid,benzenoid,esters,alcohols,and aldehydes in descending order of content.Among them,alkanes and alkenes were the categories with higher content,with an average proportion of 42.43%and 28.61%,respectively,but they had no obvious odor characteristics.19 active substances of odor were further screened by the odor activity value(OAV).Among them,the characteristic substances of odor that can be perceived by the odor activity value(OAV>1)were all terpenoids includingα-ionone,β-ionone,trans-β-ionone and farnesene,among which ionone endowed water lilies with woody and sweet fragrance,while farnesene endowed water lilies with apple and typical floral fragrance.It is indicated that the five varieties of water lilies including‘Blue Bird’,‘Kara Sunshine’,‘Golden Country’,‘Ruby’,and‘Pink Diamond’screened based on the odor activity value are potential elite varieties for landscaping use and processing product.

关 键 词：睡莲 挥发物 气味活性成分 HS-SPME-GC-MS 品种筛选 

分 类 号：S682.32[农业科学—观赏园艺]