细胞分裂周期相关蛋白5(CDCA5)在人类肿瘤中致癌作用的泛癌分析  

A Pan-Cancer Analysis of the Oncogenic Role of Cell Division Cycle-Associated Protein 5(CDCA5)in Human Tumors

作　　者：陈铭[1] 丁勇 高振国 CHEN Ming;DING Yong;GAO Zhenguo(Department of Otorhinolaryngology,First Affiliated Hospital(Chongqing Hospital)of Guangzhou University of Traditional Chinese Medicine(Beibei District Hospital of Traditional Chinese Medicine,Chongqing),Chongqing 400700,China;Nanchong Central Hospital,Affiliated Hospital of North Sichuan Medical College,Nanchong Sichuan 637100,China)

机构地区：[1]广州中医药大学第一附属医院重庆医院(重庆市北碚区中医院)耳鼻咽喉科,重庆400700 [2]川北医学院附属南充市中心医院,四川南充637100

出　　处：《西南大学学报（自然科学版）》2024年第6期98-108,共11页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市中医药重点学科建设项目.

摘　　要：CDCA5高表达已被发现在多种肿瘤中具有致癌作用.运用生物信息学方法探讨CDCA5在泛癌中的表达特征,通过TCGA和GTEX数据库获得CDCA5在肿瘤样本和癌旁样本中的表达情况,从基因表达、生存分析、基因变异和免疫浸润等方面探讨了CDCA5在不同肿瘤发病机制中的作用;通过GSCA(Gene Set Cancer Analysis)分析CDCA5表达与药物IC_(50)(半数抑制浓度)的关系并用免疫组化验证了该基因;通过STRING和GEPIA2数据库获取与CDCA5相关的基因并进行KEGG(Kyoto Encyclopedia of Genes and Genomes)富集分析.结果表明:CDCA5在大多数肿瘤中高表达,且与部分肿瘤患者的预后存在明显的相关性.CDCA5表达与肿瘤浸润免疫细胞(TIICs)显著相关并且TIICs标记物表现出不同的免疫浸润模式.拷贝数变异(CNV)与CDCA5的表达呈正相关,甲基化与CDCA5的表达呈负相关.IC_(50)数据显示多种药物可显著抑制CDCA5的表达.免疫组化实验证实了CDCA5在肝恶性肿瘤和结肠恶性肿瘤中具有促癌作用,可作为肿瘤治疗的生物标志物,为肿瘤治疗提供了一种新的研究方向.High CDCA5 expression has been found to have oncogenic effects in a variety of tumors.In this study,we aimed to explore the expression characteristics of CDCA5 gene in pan-cancer through bioinformatics methods.The expression of CDCA5 in tumor samples and paracancerous samples were obtained from the Cancer Genome Atlas(TCGA)and the Genotype Tissue Expression(GTEX)databases.The role of CDCA5 in the pathogenesis of different tumors was explored from the aspects of gene expression,survival analysis,gene variation and immune infiltration.The relationship between CDCA5 expression and IC_(50)(Half inhibitory concentration)was analyzed by GSCA(Gene Set Cancer Analysis).Moreover,we validated the gene using immunohistochemistry.The results show that CDCA5 highly expressed in most of tumors examined in this study.Elevated expression of CDCA5 was closely associated with poor prognosis of patients with these types of tumors.There was a significant correlation between CDCA5 expression and TIICs.Moreover,markers of TIICs exhibited distinct patterns of CDCA5 associated immune infiltration.CNV is thought to demonstrate a positive correlation with CDCA5 expression.Conversely,methylation has a negative correlation with CDCA5 expression.IC_(50) data showed that multiple drugs could significantly inhibited CDCA5 expression.Immunohistochemical experiments confirmed a cancer promoting role of CDCA5 in liver cancer and colon cancer,which can be used as a biomarker for cancer immunologic infiltration,and providing a new way of approach for cancer treatment.

关 键 词：细胞分裂周期相关蛋白5 泛癌分析 免疫浸润 肿瘤 

分 类 号：Q786[生物学—分子生物学] R730[医药卫生—肿瘤]