Inhibition of EGFR attenuates EGF-induced activation of retinal pigment epithelium cell via EGFR/AKT signaling pathway  

作　　者：Yu-Sheng Zhu Si-Rui Zhou Hui-Hui Zhang Tong Wang Xiao-Dong Chen 

机构地区：[1]Faculty of Life Sciences and Medicine,Northwest University,Xi’an 710069,Shaanxi Province,China [2]First Affiliated Hospital of Northwest University,Northwest University,Xi’an 710069,Shaanxi Province,China [3]Department of Ophthalmology,Xi’an No.1 Hospital,Xi’an 710002,Shaanxi Province,China [4]Shaanxi Institute of Ophthalmology,Shaanxi Provincial Key Lab of Ophthalmology,Clinical Research Center for Ophthalmology Diseases of Shaanxi Province,Xi’an 710002,Shaanxi Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2024年第6期1018-1027,共10页国际眼科杂志（英文版）

基　　金：Supported by the Natural Science Foundation of Shaanxi Province,China(No.2022JM-521).

摘　　要：AIM:To explore the effect of epidermal growth factor receptor(EGFR)inhibition by erlotinib and EGFR siRNA on epidermal growth factor(EGF)-induced activation of retinal pigment epithelium(RPE)cells.METHODS:Human RPE cell line(ARPE-19 cells)was activated by 100 ng/mL EGF.Erlotinib and EGFR siRNA were used to intervene EGF treatment.Cellular viability,proliferation,and migration were detected by methyl thiazolyl tetrazolium(MTT)assay,bromodeoxyuridine(BrdU)staining assay and wound healing assay,respectively.EGFR/protein kinase B(AKT)pathway proteins and N-cadherin,α-smooth muscle actin(α-SMA),and vimentin were tested by Western blot assay.EGFR was also determined by immunofluorescence staining.RESULTS:EGF treatment for 24h induced a significant increase of ARPE-19 cells’viability,proliferation and migration,phosphorylation of EGFR/AKT proteins,and decreased total EGFR expression.Erlotinib suppressed ARPE-19 cells’viability,proliferation and migration through down regulating total EGFR and AKT protein expressions.Erlotinib also inhibited EGF-induced an increase of proliferative and migrative ability in ARPE-19 cells and clearly suppressed EGF-induced EGFR/AKT proteins phosphorylation and decreased expression of N-cadherin,α-SMA,and vimentin proteins.Similarly,EGFR inhibition by EGFR siRNA significantly affected EGF-induced an increase of cell proliferation,viability,and migration,phosphorylation of EGFR/AKT proteins,and up-regulation of N-cadherin,α-SMA,and vimentin proteins.CONCLUSION:Erlotinib and EGFR-knockdown suppress EGF-induced cell viability,proliferation,and migration via EGFR/AKT pathway in RPE cells.EGFR inhibition may be a possible therapeutic approach for proliferative vitreoretinopathy(PVR).

关 键 词：ERLOTINIB epidermal growth factor receptor protein kinase B epithelial-mesenchymal transition retinal pigment epithelium cell 

分 类 号：R774.1[医药卫生—眼科]