Niosome-encapsulated auraptene reduced the mRNA expression of VEGF-A and PDGFs genes in human retinaderived RPE cell line  

作　　者：Akram Vahidi Teymoor Khosravi Farzad Dastaviz Mehdi Sheikh Arabi Ayyoob Khosravi Morteza Oladnabi 

机构地区：[1]Student Research Committee,Golestan University of Medical Sciences,Gorgan 4934174611,Iran [2]Department of Medical Nanotechnology,Faculty of Advanced Medical Technologies,Golestan University of Medical Sciences,Gorgan 4934174611,Iran [3]Stem Cell Research Center,Golestan University of Medical Sciences,Gorgan 4934174611,Iran [4]Department of Molecular Medicine,Faculty of Advanced Medical Technologies,Golestan University of Medical Sciences,Gorgan 4934174611,Iran [5]Gorgan Congenital Malformations Research Center,Golestan University of Medical Sciences,Gorgan 4934174611,Iran

出　　处：《International Journal of Ophthalmology(English edition)》2024年第6期1028-1035,共8页国际眼科杂志（英文版）

基　　金：Supported by Golestan University of Medical Sciences(No.111294).

摘　　要：AIM:To evaluate the effect of auraptene(AUR)treatment in forms of free and encapsulated in niosome nanoparticles by investigating the mRNA expression level of vascular endothelium growth factor(VEGF)-A and platelet-derived growth factors(PDGFs)in human retinal pigment epithelium(RPE)cell line.METHODS:Niosome nanocarriers were produced using two surfactants Span 60 and Tween 80.RPE cell line was treated with both free AUR and niosome-encapsulated.Optimum dosage of treatments was calculated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Expression of VEGF-A and PDGF-A,PDGF-B,PDGF-C,PDGF-D genes was measured after total RNA extraction and cDNA synthesis,using real-time polymerase chain reaction(RT-PCR).RESULTS:The highest entrapment efficiency(EE)was achieved by Span 60:cholesterol(1:1)with 64.3%.The half maximal inhibitory concentration(IC50)of free and niosome-encapsulated AUR were 38.5 and 27.78µg/mL,respectively.Release study revealed that niosomal AUR had more gradual delivery to the cells.RT-PCR results showed reduced expression levels of VEGF-A,PDGF-A,PDGF-B,PDGF-C,and PDGF-D after treatment with both free and niosomal AUR.CONCLUSION:Niosomal formulation of Span 60:cholesterol(1:1)is an effective drug delivery approach to transfer AUR to RPE cells.VEGF-A,PDGF-A,PDGF-B,PDGF-C,and PDGF-D are four angiogenic factors,inhibiting which by niosomal AUR may be effective in age-related macular degeneration.

关 键 词：auraptene NIOSOME age-related macular degeneration angiogenesis NANOCARRIER 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]