云木香内生细菌的分离鉴定及其对大丽轮枝菌的抑制作用  

Isolation,identification of endophytic bacteria from Aucklandia costus and its inhibitory effect on Verticillium dahliae

作　　者：金利容[1] 许冬[1] 王玲[1] 丛胜波[1] 杨妮娜[1] 李文静 尹海辰 黄薇[1] 武怀恒[1] 万鹏[1] JIN Lirong;XU Dong;WANG Ling;CONG Shengbo;YANG Nina;LI Wenjing;YIN Haichen;HUANG Wei;WU Huaiheng;WAN Peng(Key Laboratory of Integrated Pest Management on Crops in Central China,Ministry of Agriculture and Rural Affairs,Hubei Key Laboratory of Crop Disease,Insect Pests and Weeds Control,Institute of Plant Protection,Soil and Fertilizer Research,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]农业农村部华中作物有害生物综合治理重点实验室,农作物重大病虫草害防控湖北省重点实验室,湖北省农业科学院植保土肥研究所,武汉430064

出　　处：《植物保护》2024年第3期46-54,共9页Plant Protection

基　　金：农业农村部华中作物有害生物综合治理重点实验室开放基金(2021ZTSJJ2);湖北省农业科技创新中心项目(2021-620-000-001-011)。

摘　　要：从药用植物云木香中分离得到41株内生细菌,采用细菌16S rRNA基因测序的方法对这些菌株进行鉴定,结果显示,这些云木香内生细菌分别属于6个属,其中假单胞菌属Pseudomonas和拉恩氏菌属Rahnella为优势菌属。采用对峙培养方法对这些菌株进行筛选,发现其中12株内生细菌对大丽轮枝菌Verticillium dahliae具有抑制作用,其中有2株属于拉恩氏菌属Rahnella,7株属于假单胞菌属Pseudomonas,3株属于沙雷氏菌属Serratia。12株内生细菌对棉花黄萎病的温室防效评估结果表明,假单胞菌属菌株R1-6、R1-13和S1-2对棉花黄萎病的温室防效达到55%以上,具有潜在的生产利用价值。Forty-one strains of endophytic bacteria were isolated from the medicinal plant Aucklandia costus,and the 16S rRNA gene sequencing method was employed for their identification.The results showed that the endophytic bacteria belonged to six genera,with Pseudomonas and Rahnella being the predominant ones.Subsequently,these isolates were screened via confrontation culture,where 12 isolates exhibited inhibitory effects against Verticillium dahliae,among which two isolates were identified as Rahnella spp.,seven isolates as Pseudomonas spp.,and three as Serratia spp..In a greenhouse experiment evaluating the effectiveness of these 12 isolates against cotton Verticillium wilt,showing that the control effect of R1-6,R1-13,and S1-2 belonging to Pseudomonas exceeded 55%,indicating their potential utility in production.

关 键 词：云木香 内生细菌 鉴定 大丽轮枝菌 抑菌活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S435.621[农业科学—植物保护]