无鞘流毛细管电泳-电喷雾串联质谱用于面粉中荧光增白剂的高灵敏检测  

Highly sensitive detection of fluorescent whitening agents in flour using sheathless capillary electrophoresis-electrospray ionization-tandem mass spectrometry

作　　者：王安平 陈楚诗 杨巾栏 杨丽[1] WANG Anping;CHEN Chushi;YANG Jinlan;YANG Li(Northeast Normal University,Changchun 130024,China)

机构地区：[1]东北师范大学,吉林长春130024

出　　处：《色谱》2024年第6期590-598,共9页Chinese Journal of Chromatography

基　　金：国家自然科学基金(22074014);吉林省高等教育教学改革研究课题(131003552).

摘　　要：食品中残留的过量荧光增白剂(FWAs)对人体健康存在潜在威胁,因此,开发准确、高灵敏的FWAs检测方法在食品安全监测方面具有重要意义。本工作提出了利用无鞘流接口的毛细管电泳-电喷雾串联质谱(CE-ESI-MS/MS)的方法,以实现面粉样品中6种FWAs的高灵敏检测。实验采用超声辅助液相萃取法进行样品前处理,以减小复杂样品中基质效应的干扰。以氯仿-甲醇(3∶2,v/v)溶液作为萃取剂,在30℃下对样品中的FWAs进行萃取。萃取完成后,经离心、氮气吹干后用氯仿-甲醇(1∶4,v/v)复溶后检测。无鞘流CE-ESI-MS/MS方法采用正离子(ESI+)和多反应监测(MRM)模式,利用二级质谱对6种目标物同时进行定性定量分析,从而提高方法的检测通量和灵敏度。结果显示,本方法具有较宽的线性范围、良好的线性关系和较低的方法检出限(0.04~0.67 ng/g),在实际样品中3个水平下的加标回收率良好(86.2%~103.7%),日间和日内重复性(RSD)分别不大于11.5%和10.2%。上述研究表明,本方法适用于复杂基质中多种FWAs的准确、高灵敏检测,在面粉样品的质量评估和FWAs的污染监控方面具有潜在的应用价值。Fluorescent whitening agents(FWAs)are dyes that emit visible blue or blue-purple fluorescence upon ultraviolet-light absorption.Taking advantage of light complementarity,FWAs can compensate for the yellow color of many substances to achieve a whitening effect;thus,they are used extensively in various applications.FWAs are generally stable,but their presence in the environment can lead to pollution and accumulation in the body through the food chain.Recent studies have revealed that some types of FWAs,such as coumarin-based FWAs,may exhibit photo-induced mutagenic effects that can trigger allergic reactions in humans and even pose carcinogenic risks.Hence,the development of an accurate and highly sensitive method for detecting FWAs in food-related samples is a crucial endeavor.Owing to the high polarity and structural similarity of FWAs,the accurate determination of these substances in complex food samples requires an analytical method that offers both efficient separation and sensitive detection.Capillary electrophoresis(CE)exhibits essential features such as high separation efficiency,short analysis times,very small sample injection requirements,minimal use of organic solvents,and simple operation.Thus,it is often used as an effective alternative to liquid chromatographic techniques.Over the past few decades,electrospray ionization mass spectrometry(ESI-MS)has been utilized as a highly sensitive and accurate detection method in numerous chemical analytical fields because it enables the analysis of molecular structures.By combining the high separation efficiency of CE with the high sensitivity of ESI-MS,a powerful tool for identifying and quantifying trace amounts of FWAs in food samples may be obtained.In this study,we present a method based on sheathless CE coupled with electrospray ionization tandem mass spectrometry(ESI-MS/MS)for the simultaneous detection of six trace FWAs in flour.In the proposed method,the CE separation device is directly coupled to the mass spectrometer through a sheathless interface with

关 键 词：毛细管电泳 无鞘流电喷雾电离串联质谱 荧光增白剂 面粉 

分 类 号：O658[理学—分析化学]