Assessment of spermatogenesis in camels via seminiferous tubule staging and testosterone profiling  

作　　者：Saif Ullah Abdul Quddus Quanwei Wei Wael Ennab Sheeraz Mustafa Jiahao Sun Rahmani Mohammad Malyar Abdul Baseer Dagan Mao Fangxiong Shi 

机构地区：[1]College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [2]Faculty of Veterinary and Animal Sciences,Lasbela University of Agriculture Water and Marine Sciences,Balochistan,Pakistan [3]Faculty of Veterinary and Animal Sciences Ziaddin University Karachi,Pakistan

出　　处：《Asian pacific Journal of Reproduction》2024年第3期133-142,共10页亚太生殖杂志（英文版）

摘　　要：Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel reproduction.Methods:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local abattoir.The histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a microscope.Stereological analysis,germ cell identification,and assessment of seminiferous tubules and maturation were conducted.Testosterone assay was performed by radioimmunoassay using blood samples collected at regular intervals.Results:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular processes.Acrosomal migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome biogenesis.Spermatids and mature sperm cells were present when spermatogenesis phases were examined.An analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal characteristics.Moreover,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal dynamics.Conclusions:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal biogenesis.It also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation.

关 键 词：SPERMATOGENESIS CAMEL Testosterone profile Morphological staging 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]