圈养野生动物粪便及生境耐药菌分布状况研究  

Distribution of Resistant Bacteria in Captive Wild Animals and Habitats

作　　者：吕彩云[1] 郑亚文 王紫薇 谢文婷 杨岩松 张迁 张妍 贾情娜 李群 LYU Caiyun;ZHENG Yawen;WANG Ziwei;XIE Wenting;YANG Yansong;ZHANG Qian;ZHANG Yan;JIA Qingna;LI Qun(School of Life and Environmental Science,Huangshan University,Huangshan 245041,China;South Anhui National Wildlife Rescue Center,Huangshan 245041,China)

机构地区：[1]黄山学院生命与环境科学学院,安徽黄山245041 [2]皖南国家野生动物救护中心,安徽黄山245041

出　　处：《环境科学与技术》2024年第2期116-124,共9页Environmental Science & Technology

基　　金：安徽省教育厅重点项目(2023AH051375);国家重点实验室开放课题项目(SKLSGB-ORP202106);企事业合作项目(hxkt2023016,hxkt2023094);安徽省大学生创新训练项目(202210375097,S202310375051)。

摘　　要：为探究圈养野生动物粪便及生境抗生素耐药菌分布情况,该研究利用不同抗生素选择性平板采集7种野生动物粪样和生境水土样共54份样本,进行耐药菌的筛选和检出率计算与分析。结果显示,野生动物周围环境样本耐药菌检出率与粪样耐药菌检出率统计结果基本类似,其中粪样耐药菌检出率介于0~73.36%之间,生境中水样耐药菌检出率介于0~65.69%之间,土样耐药菌检出率介于0~82.47%之间。野生动物粪样存在不同程度细菌耐药现象,并有明显多重耐药性。野生动物中氨苄青霉素耐药性最为严重,其次为氟苯尼考抗生素。该研究可为中国野生动物自然环境细菌耐药性监测工作奠定基础。To explore the distribution of antibiotic-resistant bacteria in captive wild animals and habitats,a total of 54 fecal samples and habitat samples were collected from 7 wild animals for the detection and analysis of antibiotic resistant bacteria by different antibiotics selective tablet.The results showed that wild animal fecal samples of drug-resistant bacteria detection rate is between 0~73.36%.Habitat in water samples of drug-resistant bacteria detection rate between 0~65.69%,soil samples of drug-resistant bacteria detection rate between 0~82.47%.Wild animal fecal samples are different degree of bacterial drug resistant,and have obvious multiple drug resistance.The detection rate of wildlife habitat sample drug-resistant bacteria is similar with the detection rate of fecal samples drug-resistant bacteria.Ampicillin resistance is most serious in wildlife,followed by florfenicol.The study would lay the foundation for surveillance of natural environment bacterial drug resistance.

关 键 词：野生动物 粪便 生境 抗生素 耐药菌 

分 类 号：X172[环境科学与工程—环境科学] S852.6[农业科学—基础兽医学]