饲料中添加丙氨酰-谷氨酰胺对大鳞副泥鳅生长性能、血清生化指标、肝脏抗氧化能力以及肠道消化酶活性和形态结构的影响  

Effects of Dietary Alanyl⁃Glutamine Supplementation on Growth Performance,Serum Biochemical Indices,Liver Antioxidant Capacity,Intestinal Digestive Enzyme Activities and Morphological Structure of Paramisgurnus dabyranus

作　　者：李佑杰 陈琪 袁志文 潘杰 李雅萍 张亚洲 周秋白[1,2] 王自蕊 LI Youjie;CHEN Qi;YUAN Zhiwen;PAN Jie;LI Yaping;ZHANG Yazhou;ZHOU Qiubai;WANG Zirui(College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China;Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding,Nanchang 330045,China)

机构地区：[1]江西农业大学动物科学技术学院,南昌330045 [2]南昌市特色水生生物营养生理与健康养殖重点实验室,南昌330045

出　　处：《动物营养学报》2024年第5期3219-3230,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家现代农业产业技术体系(CARS-46);江西省教育厅重点项目(GJJ210404);抚州市“揭榜挂帅”项目(抚科计字[2022]8号)。

摘　　要：本试验旨在探讨丙氨酰-谷氨酰胺(Ala-Gln)对大鳞副泥鳅生长性能、血清生化指标、肝脏抗氧化能力以及肠道消化酶活性和形态结构的影响。选用初始体质量为(4.07±0.01) g的大鳞副泥鳅幼苗1 440尾,随机分为6组,每组4个重复,每个重复60尾,各组分别饲喂添加0(对照)、0.25%、0.50%、0.75%、1.00%、1.25%Ala-Gln的饲料。常规饲养8周。结果表明:饲料中添加不同水平Ala-Gln显著影响大鳞副泥鳅的增重率、特定生长率和蛋白质效率,其中,与对照组相比,0.25%~1.00%组均显著升高(P<0.05),与对照组相比,0.75%组达到最大值;与对照组相比,饲料中添加0.75%Ala-Gln显著提高大鳞副泥鳅肝体比和脏体比(P<0.05)。0.75%组血清总抗氧化能力(T-AOC)和免疫球蛋白M(IgM)含量显著提高(P<0.05)。与对照组相比,在饲料中添加0.25%~1.00%Ala-Gln能显著提高大鳞副泥鳅肝脏超氧化物歧化酶(SOD)活性(P<0.05),添加0.50%~1.00%Ala-Gln能显著提高肝脏过氧化氢酶(CAT)活性(P<0.05),添加0.50%Ala-Gln能显著提高肝脏谷胱甘肽(GSH)含量(P<0.05),添加0.75%、1.00%Ala-Gln能显著提高肝脏谷胱甘肽过氧化物酶(GPX)活性(P<0.05),其中,SOD和GPX活性在1.00%组达到最大,CAT活性在0.75%组最大,GSH活性在0.50%组最大;各组肝脏MDA含量在添加Ala-Gln后均显著降低(P<0.05),其中0.75%组最低。在肠道消化酶中,与对照组相比,0.75%和1.00%组胰蛋白酶活性显著升高(P<0.05),且在0.75%组达到最大。与对照组相比,饲料中添加0.75%~1.25%Ala-Gln能显著提高大鳞副泥鳅肠道绒毛高度(P <0.05),添加0.25%~1.25%Ala-Gln能显著提高绒毛宽度(P<0.05),均在添加量为0.75%时达到最大。综上所述,饲料中添加Ala-Gln能促进大鳞副泥鳅生长,提高抗氧化能力和免疫能力,改善肠道形态,促进消化吸收,其中以0.75%添加量效果最佳。This experiment was conducted to investigate the effects of alanyl⁃glutamine(Ala⁃Gln)on growth performance,serum biochemical indices,liver antioxidant capacity,intestinal digestive enzyme activities and morphological structure of Paramisgurnus dabyranus.A total of 1440 seedlings with an initial body weight of(4.07±0.01)g were randomly divided into 6 groups with 4 replicates per group,and with 60 fish in each rep⁃licate.Diets containing 0(control),0.25%,0.50%,0.75%,1.00%and 1.25%Ala⁃Gln were prepared,re⁃spectively.The animals were fed for 8 weeks.Results showed that the weight gain rate(WGR),specific growth rate(SGR)and protein efficiency ratio of Paramisgurnus dabyranus which were significantly increased in 0.25%to 1.00%groups compared with control group(P<0.05),and reached the maximum value in 0.75%group.The hepatosomatic ratio and viscerosomatic ratio of Paramisgurnus dabyranus were significantly increased in 0.75%group compared with control group(P<0.05).Serum total antioxidant capacity(T⁃AOC)and immunoglobulin M(IgM)content in 0.75%group were significantly higher than those in control group(P<0.05);dietary supplementation of 0.25%to 1.00%Ala⁃Gln significantly increased liver superoxide dis⁃mutase(SOD)activity(P<0.05),dietary supplementation of 0.50%to 1.00%Ala⁃Gln significantly in⁃creased catalase(CAT)activity(P<0.05),supplementation with 0.50%Ala⁃Gln significantly increased glu⁃tathione(GSH)content(P<0.05),supplementation with 0.75%and 1.00%Ala⁃Gln significantly increased glutathione peroxidase(GPX)activity(P<0.05).The activities of SOD and GPX were the highest in 1.00%group,and the activity of CAT was the highest in 0.75%group.The GSH content was the highest in 0.50%group(P<0.05).The MDA content in liver of all groups was significantly decreased after adding Ala⁃Gln(P<0.05),and 0.75%group was the lowest.In intestinal digestive enzymes,trypsin activity in 0.75%and 1.00%groups was significantly increased compared with control group(P<0.05),and reached the maximum in 0.75%group.Com

关 键 词：丙氨酰-谷氨酰胺 大鳞副泥鳅 生长性能 抗氧化能力 肠道形态结构 

分 类 号：S963[农业科学—水产养殖]