金合欢素对幽门螺杆菌感染的胃上皮GES-1细胞凋亡的抑制作用  

Effect of Acacetin on Inhibition of Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cell Line GES-1

作　　者：姚奇汐 李自宇 康厚乐 贺欣 康敏 YAO Qi-xi;LI Zi-yu;KANG Hou-le;HE Xin;KANG Min(Department of Gastroenterology,The Affiliated Hospital of Southwest Medical University,Southwest Medical University,Luzhou 646000,China;Department of Orthopaedics,The Affiliated Hospital of Southwest Medical University,Southwest Medical University,Luzhou 646000,China;Luzhou People′s Hospital,Luzhou 646000,China)

机构地区：[1]西南医科大学附属医院消化内科,646000 [2]西南医科大学附属医院骨科,646000 [3]泸州市人民医院,646000

出　　处：《现代消化及介入诊疗》2024年第3期307-311,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：西南医科大学附属医院博士科研启动基金资助(21037)。

摘　　要：目的研究金合欢素对幽门螺旋杆菌(Hp)感染GES-1细胞凋亡的保护作用及潜在的作用机制。材料和方法体外用Hp和金合欢素处理GES-1细胞,CCK-8法检测细胞活力,创面愈合法评估细胞迁移及修复能力的变化,流式细胞术分析细胞凋亡率,western blots法检测凋亡相关蛋白的表达水平。结果Hp可诱导GES-1细胞活力下降,抑制细胞迁移,使细胞凋亡比率增加,同时增加Bax、cle-caspase3的表达水平。而金合欢素处理可增强细胞活力,抑制Hp感染所致的细胞凋亡水平,并下调Bax、cle-caspase3的表达。讨论与结论金合欢素能增强GES-1细胞活性,通过抑制Hp感染的GES-1细胞凋亡,从而对胃黏膜上皮细胞具有保护作用。Objective This study aims to elucidate the protective role of Acacetin against apoptosis in HP-infected GES-1 cells and to delineate its potential underlying mechanisms.Materials and Methods GES-1 cells were subjected to in vitro treatment with HP and Acacetin.Cell viability was assessed utilizing the CCK-8 assay,alterations in cell migration and healing capacities through the wound healing assay,rates of apoptosis via flow cytometry,and expression levels of apoptosis-associated proteins through western blot analysis.Results HP infection led to a diminution in GES-1 cell viability,a suppression of cell migration,an augmentation in the rate of apoptosis,and an increase in the expression levels of Bax and cle-caspase3.Conversely,treatment with Acacetin was found to enhance cell viability,mitigate apoptosis induced by HP infection,and modulate the expression of apoptosis proteins by downregulating Bax and cle-caspase3.Discussion and Conclusion Acacetin significantly improves GES-1 cell vitality and inhibits apoptosis in HP-infected GES-1 cells,thereby offering a protective effect on gastric mucosal epithelial cells.

关 键 词：胃上皮GES-1细胞株 幽门螺旋杆菌 感染 金合欢素 细胞凋亡 

分 类 号：R573[医药卫生—消化系统]