通络活血散对木瓜蛋白酶致炎大鼠髌骨的影响  

The effects of Tongluo Huoxue San on patella of rats with papain-induced inflammation

作　　者：夏华玲 谢文 秦晓飞 潘琨琨 赵新杰 薛素民 XIA Hualing

机构地区：[1]河南省洛阳正骨医院(河南省骨科医院),河南洛阳471002

出　　处：《中医临床研究》2024年第11期14-20,共7页Clinical Journal Of Chinese Medicine

基　　金：2020-2021年河南省中医药科学研究专项重点课题(20-21ZY1065)。

摘　　要：目的:探讨通络活血散对木瓜蛋白酶致炎大鼠髌骨的影响。方法:使用木瓜蛋白酶诱导大鼠髌骨软化症动物模型,通过红外热成像、显微镜检和免疫组化技术研究大鼠体温、髌骨表面形态、髌骨组织,以及滑膜中肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)和转化生长因子(Transforming Growth Factor,TGF)-β1的变化。结果:①红外热成像显示,通络活血散高剂量组、通络活血散中剂量组红色最深,空白组、双氯芬酸二乙胺乳胶剂组、通络活血散低剂量组红色次之,模型组红色最浅。②金相显微镜观察发现,空白组大鼠髌骨表面平滑,凹凸排列均匀;模型组大鼠髌骨表面粗糙,多裂隙,排列不规则;双氯芬酸二乙胺乳胶剂组大鼠髌骨表面粗糙,多细小裂隙,排列不规则;通络活血散高剂量组、通络活血散中剂量组、通络活血散低剂量组大鼠髌骨表面平滑,可见凹凸,稀疏,排列不均匀,有少量细小裂隙。③空白组大鼠滑膜组织表面被覆单层滑膜细胞,髌骨组织分层清晰。模型组大鼠滑膜增生合并慢性炎症,髌骨表面不规则,有血管翳形成,表层储备细胞退变,增生层细胞明显增生,分层结构紊乱。通络活血散高剂量组、通络活血散中剂量组滑膜表面被覆单层滑膜细胞,有轻微增生,髌骨表面表层储备细胞增生,分层基本清晰。通络活血散低剂量组和双氯芬酸二乙胺乳胶剂组大鼠滑膜组织增生合并少量炎细胞浸润,髌骨表面表层储备细胞局部退变,增生层细胞明显增生。④在TNF-α免疫组化染色阳性率方面,模型组、双氯芬酸二乙胺乳胶剂组,以及通络活血散高剂量组、通络活血散中剂量组、通络活血散低剂量组均高于空白组(P<0.05);模型组高于双氯芬酸二乙胺乳胶剂组以及通络活血散高剂量组、通络活血散中剂量组、通络活血散低剂量组(P<0.05);通络活血散低剂量组高于双氯芬酸二乙胺乳胶剂组�Objective:To observe the effects of Tongluo Huoxue San(通络活血散)on patella of rats with papain-induced inflammation.Methods:Rat models of patellar malacia induced by papain were used to investigate the changes of body temperature,patellar surface morphology,patellar tissue,and TNF-αand TGF-β1 in synovium by infrared thermal imaging,microscopy,and immunohistochemical techniques.Results:①Infrared thermal imaging showed that the high and medium dose groups of Tongluo Huoxue San had the deepest red color,followed by the blank group,the diclofenac diethylamine emulsion group,and the low dose group of Tongluo Huoxue San,and the red color in the model group was the lightest.②Metallographic microscope observation showed that the patella of the rats in the blank group had a smooth surface and even arranged uniformly.The patella surface of the rats in the model group was rough,with multiple fissures and irregular arrangement.The patella surface of rats was rough,with many small cracks and irregular arrangement in the diclofenac diethylamine emulsion group.The surface of the patella of rats was smooth,uneven,sparse,uneven arrangement,and a few small cracks in the high,medium and low dose groups of Tongluo Huoxue San.③The synovial tissue surface of the rats in the blank group was covered with a single layer of synovial cells,and the patella tissue was clearly stratified.Synovial hyperplasia,chronic inflammation,irregular patella surface,pannus formation,degeneration of surface reserve layer cells,obvious proliferation of proliferative layer cells,and disordered layered structure were observed in the model group.The synovial surface of rats in the high and medium dose groups of Tongluo Huoxue San was covered with a single layer of synovial cells with slight hyperplasia.The cells of the reserve layer on the patellar surface were proliferated,and the stratification was basically clear.The synovial tissue of the rats in the low-dose group of Tongluo Huoxue San and the diclofenac diethylamine emulsion group was hyp

关 键 词：通络活血散 髌骨软化症 木瓜蛋白酶 免疫组化 金相显微观察 

分 类 号：R284.5[医药卫生—中药学]