冬荪多糖对免疫抑制小鼠免疫功能的影响  

Effects of Polysaccharides from Phallus inpudicu on the Immune Function of Immunosuppressed Mice

作　　者：杨克铃 彭梅 杨小生 杨娟 YANG Keling;PENG Mei;YANG Xiaosheng;YANG Juan(School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,China;Natural Products Research Center of Guizhou Province,Guizhou Medical University,Guiyang 550014,Guizhou,China;State Key Laboratory of Functions and Applications of Medcial University,Guiyang 550014,Guizhou,)

机构地区：[1]贵州医科大学药学院,贵州贵阳550025 [2]贵州医科大学贵州省天然产物研究中心,贵州贵阳550014 [3]省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014

出　　处：《食品研究与开发》2024年第11期30-38,共9页Food Research and Development

基　　金：贵州省科技计划项目(黔科合服企[2020]4013);贵州省发展和改革委员会(黔财建[2019]303);贵州省高等学校特色重点实验室建设任务(黔教合KY字[2020]018)。

摘　　要：冬荪是一种珍稀食用菌,为高值化利用冬荪资源,探究冬荪菌托多糖和子实体多糖对免疫抑制小鼠免疫功能和肠道菌群的影响,综合评价其免疫调节活性。结果表明,不同剂量冬荪菌托多糖组和子实体多糖组小鼠全血白细胞数和脾脏指数均升高(P<0.05,P<0.01或P<0.001);血清中肿瘤坏死因子α、干扰素γ水平和乳酸脱氢酶、酸性磷酸酶活力显著提高(P<0.05,P<0.01或P<0.001);脾脏丙二醛含量显著降低(P<0.05或P<0.001),过氧化氢酶活力显著升高(P<0.01或P<0.001);脾脏组织中淋巴细胞数量增多,淋巴细胞排列得到改善。冬荪菌托多糖和冬荪子实体多糖可促进短链脂肪酸生成,改变免疫抑制小鼠肠道菌群的组成,增加拟杆菌门(Bacteroidetes)相对丰度,减少厚壁菌门(Firmicutes)相对丰度。Phallus inpudicu is a kind of rare edible fungus.In order to increase the value of the resources of P.inpudicu,this study investigated the effects of polysaccharides from P.inpudicu egg tray(PIT)and fruiting body(PIF)on immunity and the gut microbiota of immunosuppressed mice,and their immunoregulatory activi-ties were comprehensively evaluated.The results showed that the total white blood cell count and spleen index in different doses of P.inpudicu PIT group and PIF group were both increased(P<0.05,P<0.01 or P<0.001),and the levels of tumor nerosis factor-α(TNF-α),interferon-γ(IFN-γ)and the activities of lactate dehydroge-nase(LDH),acid phosphatase(ACP)in serum were significantly increased(P<0.05,P<0.01 or P<0.001).The malondialdehyde(MDA)content in spleen was significantly decreased(P<0.05 or P<0.001),while the catalase(CAT)activity was significantly increased(P<0.01 or P<0.001).The number of lymphocytes in spleen tissue was increased and the arrangement of lymphocytes was improved.P.inpudicu PIT and PIF can promote the production of short-chain fatty acids,change the composition of gut microbiota in immunosuppressed mice,increase the relative abundance of Bacteroidetes,and decrease the relative abundance of Firmicutes.

关 键 词：冬荪 菌托多糖 子实体多糖 免疫调节 肠道菌群 

分 类 号：R28[医药卫生—中药学]