羟基磷灰石修饰碳纳米电极制备及其对抗坏血酸的测定  

Preparation of hydroxyapatite modified carbon nanoelectrode and its application in determination of antiascorbic acid

作　　者：丰明佳 钱功明[1,2] 熊苓雅 韩荻 FENG Mingjia;QIAN Gongming;XIONG Lingya;HAN Di(School of Resources and Environmental Engineering,Wuhan University of Science and Technology,Wuhan 430081,China;Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources,Wuhan 430081,China)

机构地区：[1]武汉科技大学资源与环境工程学院,武汉430081 [2]冶金矿产资源高效利用与造块湖北省重点实验室,武汉430081

出　　处：《分析试验室》2024年第5期654-659,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21705122);湖北省高等学校优秀中青年科技创新团队计划(T2021002)项目资助。

摘　　要：通过恒电位沉积法,将纳米羟基磷灰石(HAP)成功修饰到碳纳米电极(CNE)上,制备出一种能快速检测抗坏血酸(AA)的修饰电极(HAP-CNE)。采用循环伏安法(CV)和计时安培法(CA)研究了HAP-CNE电极对AA的检测,考察了多巴胺(DA)和模拟体液pH对电极检测AA的影响。扫描电镜(SEM)结果表明,HAP已经成功修饰于CNE尖端与外壁上。电化学实验表明,与CNE相比,HAP-CNE电极信噪比显著提高,同时AA在HAP-CNE上能发生氧化还原反应,电荷转移速率提高。AA浓度在1×10^(-5)~2×10^(-3)mol/L范围内与响应电流呈近似线性关系,检出限为1.3×10^(-7)mol/L。利用HAP-CNE检测AA具有广泛的pH适应性且对DA的抗干扰能力强。该电极是一种潜在原位探针,有望为生物医学研究提供新的检测方法,并成为一种能应用于活体实时检测生物小分子代谢过程的新型传感器。Nano-hydroxyapatite(HAP)was successfully modified onto carbon nanoelectrode(CNE)by potentiostatic deposition method,and a novel modified electrode(HAP-CNE)waspreparedforrapid determination of ascorbic acid(AA).Cyclic voltammetry(CV)and chronoamperometry(CA)were used to detect AA on HAP-CNE,and the effects of dopamine(DA)and pH were investigated.The results of scanning electron microscopy(SEM)showed that HAP had been modified successfully on the tip and outer wall of CNE.Electrochemical experiments showed that the signal-to-noise ratio of HAP-CNE electrode was significantly higher than that of CNE electrode.Meanwhile,the REDOX reaction of AA happened on HAP-CNE electrode,and the transfer rate increased.In addition,the AA concentration was approximately linear with the response current in the range of 1×10^(-5)-2×10^(-3)mol/L,and the detection limit was 1.3×10^(-7)mol/L.The detection of AA by HAP-CNE had a wide range of pH adaptability and strong anti-interference ability to DA.The electrode is a potential in situ probe that is expected to provide new detection methods for biomedical research and to become a novel sensor that can be applied to the real-time detection of metabolic processes of small molecules in vivo.

关 键 词：碳纳米电极 羟基磷灰石 电化学检测 抗坏血酸 

分 类 号：O657.15[理学—分析化学]