基于红色碳点/二氧化锰纳米探针荧光法检测果汁总抗氧化性  

Fluorescent assay for the determination of total antioxidant capacity of fruit juices based on red-emitting carbon dot/MnO_(2)nanoprobe

作　　者：王幸幸 李景敏 杨顺 周扬 罗林[2] 邓浩[3] 何镇熹 徐振林[2] 贾宝珠 WANG Xingxing;LI Jingmin;YANG Shun;ZHOU Yang;LUO Lin;DENG Hao;HE Zhenxi;XU Zhenlin;JIA Baozhu(College of Biological and Food Engineering,Guangdong University of Education,Guangzhou 510303,China;Guangdong Key Laboratory of Food Quality and Safety,College of Food Science,South China Agricultural University,Guangzhou 510642,China;Key Laboratory of Tropical Fruit and Vegetable Cold-chain of Hainan Province,Institute of Agro-products Processing and Design,Hainan Academy of Agricultural Sciences,Haikou 570100,China;Shenzhen Total-Test Technology Co.,Ltd.,Shenzhen 518038,China)

机构地区：[1]广东第二师范学院生物与食品工程学院,广州510303 [2]广东省食品质量安全重点实验室,华南农业大学食品学院,广州510642 [3]海南省农业科学院农产品加工设计研究所,海南省热带果蔬冷链研究重点实验室,海口570100 [4]深圳市通量检测科技有限公司,深圳518038

出　　处：《分析试验室》2024年第5期669-675,共7页Chinese Journal of Analysis Laboratory

基　　金：广东省教育厅普通高校青年创新人才项目(2022KQNCX055);海南省热带果蔬冷链研究重点实验室开放基金(HAAS2023PT0210);广东大学生科技创新培育专项资金“攀登计划”项目(pdjh2021b0368,pdjh2022b0382,pdjh2023b0386)资助。

摘　　要：以红色荧光碳点(R-CDs)与二氧化锰(MnO_(2))纳米片为荧光探针,构建了一种灵敏、快速、简便的荧光分析方法,并用于测定食物总抗氧化性。以抗坏血酸(AA)为模型检测靶标,采用AA当量评价食品总抗氧化性,通过单因素实验优化MnO_(2)纳米片浓度、R-CDs稀释倍数、MnO_(2)与R-CDs的作用时间及其与AA的作用时间。在最佳条件下,荧光强度与AA浓度的对数值在4.0~12μmol/L范围内呈良好的线性关系,检出限达到0.06μmol/L。该方法测定标准添加果汁样品的回收率在85.8%~113.0%之间,具有较好的灵敏度与准确性。A fluorescent assay for the determination of total antioxidant capacity(TAC)of foods was developed by using red-emitting carbon dots(R-CDs)/manganese dioxide nanosheets(MnO_(2)NSs)as nanoprobe.Ascorbic acid(AA),as a typical antioxidant,was employed as model analyte,and AA equivalents was used as the index for TAC assessment.Single-factor experiments were conducted to optimize the concentration of MnO,nanosheets,dilution factor of R-CDs,interaction time for MnO_(2)with R-CDs and AA with the nanoprobe.Under the optimal conditions,the fluorescence intensity showed a good linear relationship with AA concentration in the range of 4.0-12μmol/L,with the detection limit of 0.06μmol/L.The recoveries of the standard added juice samples ranged from 85.8%to 113.0%,demonstrating the high potential detection of TAC in food.

关 键 词：MnO_(2)纳米片 红色荧光碳点 荧光 抗氧化剂 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]