SPP1和miR-340-5p在甲状腺癌中的表达水平及预后价值  

Expression and prognostic value of SPP1 and miR⁃340⁃5p in thyroid cancer

作　　者：吕文豪 王江芬[2] 张亚芬[1,2] LÜWenhao;WANG Jiangfen;ZHANG Yafen(The Fifth Clinical Medical College of Shanxi Medical University,Taiyuan,Shanxi 030012,China;Department of Breast Surgery,Shanxi Provincial People's Hospital,Taiyuan,Shanxi 030012,China)

机构地区：[1]山西医科大学第五临床医学院乳腺外科,山西太原030012 [2]山西省人民医院乳腺外科,山西太原030012

出　　处：《热带医学杂志》2024年第4期527-531,共5页Journal of Tropical Medicine

基　　金：山西省应用基础研究计划项目(201901D111428)。

摘　　要：目的分析分泌磷蛋白1(SPP1)、微小RNA-340-5p(miR-340-5p)在甲状腺癌中的表达水平,为临床提高甲状腺癌早期诊断及预后提供帮助。方法选取山西医科大学第五临床医学院乳腺外科2018年3月-2020年5月期间收治的119例分化型甲状腺癌(DTC)患者作为研究组,根据随访期间复发转移及死亡情况分为预后良好组(n=84)和预后不良组(n=35),另选取一般资料与研究组相匹配的119例甲状腺良性病变患者作为对照组。采用Pearson法分析血清SPP1和miR-340-5p表达水平的相关性;采用多因素logistic回归分析影响DTC患者预后的相关因素;采用受试者工作特征(ROC)曲线分析血清SPP1、miR-340-5p对DTC患者预后的预测效能。结果研究组血清SPP1和miR-340-5p表达水平高于对照组,差异均有统计学意义(t=15.732、10.111,P均<0.001)。DTC患者血清SPP1与miR-340-5p表达水平成正相关(r=0.584,P<0.05)。预后不良组血清SPP1和miR-340-5p表达水平高于预后良好组,差异均有统计学意义(t=7.497、4.938,P均<0.001)。预后不良组发生淋巴结转移、TNM分期为Ⅲ+Ⅳ期的患者所占比例显著高于预后良好组,差异均有统计学意义(χ^(2)=11.297、6.802,P均<0.05)。淋巴结转移(OR=1.901)、TNM分期为Ⅲ+Ⅳ期(OR=2.178)、SPP1(OR=1.541)及miR-340-5p(OR=2.104)为DTC患者发生不良预后的危险因素(P均<0.05)。血清SPP1和miR-340-5p预测DTC患者预后状态的曲线下面积(AUC)分别为0.829(95%CI:0.749~0.892)、0.698(95%CI:0.607~0.779),二者联合预测的AUC为0.908(95%CI:0.841~0.953),二者联合优于SPP1和miR-340-5p各自单独检测(Z=2.410、3.566,P均<0.05)。结论血清SPP1和miR-340-5p表达水平与甲状腺癌的发生发展相关,二者联合检测对甲状腺癌患者预后状态具有较高的预测效能。Objective To investigate the expression of secreted phosphoprotein 1(SPP1)and microRNA⁃340⁃5p(miR⁃340⁃5p)in thyroid cancer and their relationship with prognosis.Methods From March 2018 to May 2020,119 patients with differentiated thyroid cancer(DTC)admitted to the Breast Surgery Department of The Fifth Clinical Medical College of Shanxi Medical University were selected as the study group;according to the recurrence,metastasis,and mortality during the follow⁃up period,they were grouped into a good prognosis group(n=84)and a poor prognosis group(n=35);another 119 patients with benign thyroid lesions who matched the general data with the study group were selected as the reference group.Pearson method was applied to analyze the correlation between serum SPP1 and miR⁃340⁃5p expression levels;multivariate logistic regression was applied to analyze the relevant factors affecting the prognosis of DTC patients;receiver operating characteristic(ROC)curve was applied to analyze the predictive efficacy of serum SPP1 and miR⁃340⁃5p on the prognosis of DTC patients.Results The expression levels of serum SPP1 and miR⁃340⁃5p in the study group were higher than those in the control group,the differences were statistically significant(t=15.732,10.111;all P<0.001);the expression levels of serum SPP1 and miR⁃340⁃5p in DTC patients were positively correlated(r=0.584,P<0.05);the expression levels of serum SPP1 and miR⁃340⁃5p in the poor prognosis group were higher than those in the good prognosis group,the differences were statistically significant(t=7.497,4.938;all P<0.001);the proportions of patients with lymph node metastasis and TNM stageⅢ+Ⅳin the poor prognosis group were greatly higher than those in the good prognosis group,the differences were statistically significant(χ^(2)=11.297,6.802;all P<0.05);lymph node metastasis(OR=1.901),TNM stageⅢ+Ⅳ(OR=2.178),SPP1(OR=1.541),and miR⁃340⁃5p(OR=2.104)were the risk factors for poor prognosis in DTC patients(P<0.05);the AUC of serum SPP1 and miR�

关 键 词：分泌磷蛋白1 微小RNA-340-5p 甲状腺癌 

分 类 号：R736.1[医药卫生—肿瘤]