五行音乐对孕期恐应激子代社交行为的影响及机制研究  

Effects and mechanism of five-element music on social behavior of offspring of stress-injured pregnant mice

作　　者：吴永叶 杨丽萍[1] 张振强[2] 赵佳佳 宋琦 侯俊林[1] 王耀辉 李笑琳 关徐涛[3] WU Yongye;YANG Liping;ZHANG Zhenqiang;ZHAO Jiajia;SONG Qi;HOU Junlin;WANG Yaohui;LI Xiaolin;GUAN Xutao(Traditional Chinese Medicine School,Henan University of Chinese Medicine,Zhengzhou 450046,China;College of Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046;the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450046)

机构地区：[1]河南中医药大学中医学院,郑州450046 [2]河南中医药大学中医药科学院,郑州450046 [3]河南中医药大学第一附属医院,郑州450046

出　　处：《中国比较医学杂志》2024年第4期28-34,共7页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81973596);河南省大学生创新创业训练计划项目(202210471004)。

摘　　要：目的探究五行音乐对孕期恐应激子代社交行为的影响和机制,为临床胎源性情志疾病的早期防治提供依据。方法将45只孕鼠随机分为空白组、模型组和五行音乐组。其中,模型组、五行音乐组均采用旁观电击法造模,五行音乐组在孕期的每天17:00~19:00播放五行音乐中的宫调。孕第19天对各组孕鼠采用ELISA法检测血清中促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)和皮质醇(corticosterone,CORT)的含量进行造模评价。仔鼠延续母鼠的分组,于8周龄时进行三箱社交实验观察社交行为;免疫荧光双标法检测仔鼠内侧前额叶皮层(medial prefrontal cortex,mPFC)谷氨酸能神经元活性的表达;高效液相色谱法检测仔鼠mPFC区谷氨酸(glutamate,Glu)总含量;高尔基染色观察仔鼠mPFC区神经元树突棘的变化。结果与空白组相比,模型组孕鼠血清中ACTH、CORT含量显著上升,模型组仔鼠社交交互时间和社会新奇偏好指数显著降低,谷氨酸能神经元活性、谷氨酸含量及神经元树突棘显著下降。与模型组相比,五行音乐组孕鼠血清中ACTH、CORT的含量降低及仔鼠社交行为、谷氨酸能神经元活性、谷氨酸含量及神经元树突棘的状态得到改善。结论五行音乐干预能够有效改善孕期恐应激所致子代的社交行为障碍,其机制可能与增强mPFC区谷氨酸能神经元的活性有关。Objective To investigate the effects and mechanisms of five-element music on the social behavior of the children of mothers with fear stress during pregnancy and provide a basis for the early prevention and treatment of clinical fetogenic affective disorders.Methods Forty-five pregnant mice were randomly divided into three groups:a control group,model group,and five-element music group.The model and five-element music group models were established using the bystander electric shock method.Additionally,the five-element music group was exposed to Palace Tune five-element music daily from 17:00 to 19:00 during pregnancy.On the 19th day of pregnancy,ELISA was employed to assess the levels of adrenocorticotropin(ACTH)and cortisol(CORT)in the serum of pregnant mice in each group for modeling evaluation.The offspring were subsequently grouped with their mother and underwent an 8-week-old three-box social experiment to observe their social behavior.We used the immunofluorescence double-labeling method to detect glutamatergic neuron activity in the medial prefrontal cortex(mPFC)of the offspring.High-performance liquid chromatography was employed to measure the total glutamate(Glu)content in the mPFC,while Gorky staining was used to observe changes in the dendritic spines of mPFC neurons in the offspring.Results Compared to those in the blank group,pregnant mice in the model group exhibited a significant increase in the levels of ACTH and CORT in their serum,and there was a significant decrease in the social interaction time and social novelty preference index of their offspring.There was also a significant decrease in glutamate neuron activity,glutamate content,and neuronal dendritic spine density.In contrast,compared with those in the model group,pregnant mice in the five-element music group demonstrated a reduction in the levels of ACTH and CORT in the serum,and there were improvements in the social behavior,glutamate neuron activity,glutamate content,and condition of neuronal dendritic spines in the offspring.Conclusio

关 键 词：五行音乐 孕期恐应激 子代 社交行为 谷氨酸能神经元 

分 类 号：R-33[医药卫生]