神经母细胞瘤肝及肾上腺移植模型比较  

Comparison of liver and adrenal transplantation models of neuroblastoma

作　　者：陈红霞 谭志刚 林惠然 冯露平 郑楚雅 廖文峰 曾如凤 刘锦信 卓振建 CHEN Hongxia;TAN Zhigang;LIN Huiran;FENG Luping;ZHENG Chuya;LIAO Wenfeng;ZENG Rufeng;LIU Jinxin;ZHUO Zhenjian(Laboratory Animal Center,Peking University Shenzhen Graduate School,Shenzhen 518055,China;State Key Laboratory of Chemical Oncogenomics,Shenzhen 518055;Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055)

机构地区：[1]北京大学深圳研究生院实验动物中心,广东深圳518055 [2]省部共建肿瘤化学基因组学国家重点实验室,广东深圳518055 [3]中国科学院深圳先进技术研究院,广东深圳518055

出　　处：《中国比较医学杂志》2024年第4期100-108,共9页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(82002636);深圳市自然科学基金(JCYJ20220531093213030)。

摘　　要：目的 建立神经母细胞瘤(neuroblastoma, NB)肝移植模型,并与肾上腺原位移植模型进行比较,探索模型的特征。方法 采用微量注射针将含有5×10^(5)(SK-N-SH)细胞溶液沿肝叶长轴种植于小鼠肝左叶,造模结束后检测肿瘤的生长、转移情况、相关基因表达及肿瘤组织病理学变化。结果 小鼠接种肿瘤细胞21 d后成瘤率达100%,肿瘤的生长、转移、相关基因表达及病理特征明显。结论 本文通过更为简单的手术造模方法,成功构建了一种神经母细胞瘤肝移植模型,为NB研究者今后科学实验提供了更多理想的模型选择。Objective A neuroblastoma(NB)liver transplantation model was established and compared with the adrenal orthotopic transplantation model to explore its characteristics.Methods 5×10^(5) SK-N-SH cells were implanted along the long axis of the left lobe of mouse livers with a micro-injection needle.The growth,metastasis,expression of related genes,and histopathological changes of tumors were detected after the modeling.Results The tumor formation rate in mice inoculated with tumor cells reached 100%after 21 days,and tumor growth,metastasis,related gene expression changes,and pathological characteristics were apparent.Conclusions In this study,a neuroblastoma liver transplantation model was successfully constructed via a relatively simple surgical method to provide a more suitable choice for future scientific NB experiments.

关 键 词：神经母细胞瘤 肾上腺原位移植 肝移植 疾病模型 成瘤率 

分 类 号：R-33[医药卫生]